Fig. 1.

AQP11 resides in the ER.

A. HeLa transfectants expressing HaloAQP11 were co-stained with fluorescent Halo ligands, and with antibodies against calnexin (CNX) or peroxiredoxin 3 (PRX3), to decorate AQP11, the ER and mitochondria, respectively. To label glycoproteins on the plasma membrane, cells were stained with concanavalin A-FITC (ConA) in absence of cell permeabilization, and then fixed (middle panels). Scale bar = 10 μm.

B. HeLa cells expressing AQP11mycFlag were fractionated as previously described [22,23]. Aliquots were resolved electrophoretically and stripes of the blots decorated with the indicated antibodies. Hom, total post-nuclear homogenates. Cyt, cytosol. MP, pure mitochondrial fraction. MAM, mitochondria-associated membranes. ER, endoplasmic reticulum.