AQP11 forms tetramers.
A. Aliquots of the lysates of HeLa co-transfectants expressing AQP11mycFlag and either Halo-RDEL, HaloGpx8 TM or HaloAQP11 proteins were immunoprecipitated using immobilized anti-Flag antibodies and analyzed by western blotting using anti-Halo or anti-Flag specific antibodies. IP, immunoprecipitation.
B. Lysates of HeLa cells expressing either HaloAQP11-Flag or HaloAQP8-Flag were separated through a discontinuous 5–
11% sucrose density gradient, and aliquots of each fraction subjected to reducing SDS-PAGE. The presence of the recombinant proteins was assessed by direct detection of bound fluorescent Halo ligands on the gels using a laser scanner imager.