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. 2019 Sep 12;28:101326. doi: 10.1016/j.redox.2019.101326

Fig. 3.

Fig. 3

AQP11 forms tetramers.

A. Aliquots of the lysates of HeLa co-transfectants expressing AQP11mycFlag and either Halo-RDEL, HaloGpx8 TM or HaloAQP11 proteins were immunoprecipitated using immobilized anti-Flag antibodies and analyzed by western blotting using anti-Halo or anti-Flag specific antibodies. IP, immunoprecipitation.

B. Lysates of HeLa cells expressing either HaloAQP11-Flag or HaloAQP8-Flag were separated through a discontinuous 5–

11% sucrose density gradient, and aliquots of each fraction subjected to reducing SDS-PAGE. The presence of the recombinant proteins was assessed by direct detection of bound fluorescent Halo ligands on the gels using a laser scanner imager.