Fig. 5.

AQP11 restores H₂O₂fluxes in silenced cells.HyPerERLum HeLa transfectants were silenced for AQP11 expression and then transfected with a silencing-resistant HaloAQP11.

A. Representative immunofluorescence analyses of one of the experiments averaged in panel B confirm the lower response to exogenous H₂O₂ (top panels, HyPerERLum fold changes, in orange) and higher basal oxidation of HyPer ER lumen (middle panels, basal ratio, in rainbow) in AQP11-silenced cells. Both features are rescued by re-expression of HaloAQP11, as monitored using fluorescent Halo ligands as shown in the lower panel (in white).

B. The efficiency of the entry of exogenous H₂O₂ (50 μM, top graph) and the basal oxidation state of the HyPer ER lumenal probe (bottom panel) were measured as in Fig. 4, in control or AQP11_low cells, before or after rescuing. Results are represented as percentage of H₂O₂ transported 1.5 min after of H₂O₂ addition relative to controls (Mock), and as the fold change in the basal 488/405 nm ratio (bottom). Average on ≥3 experiments ± SEM.

(For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)