Fig. 2.

mRNA expression in the small intestine of mice fed a HFD. Mice fed either LFD or HFD were euthanized after 12 weeks. The intestinal epithelium was removed, homogenized and submitted to RT-qPCR. The values obtained for each target gene were normalized with the expression levels of the housekeeping gene 18s and evaluated using Student's t test. Slc5a1 [SGLT1], Slc2a2 [GLUT2], Slc9a3 [NHE3] and Cd36 [FAT/CD36] were subjected to a parametric test, while Slc2a5 [GLUT5], Slc15a1 [PEPT1], Slc27a4 [FATP4], Npc1l1 [NPC1L1], Mttp [MTTP] and L-Fabp [L-FABP] were subjected to a non-parametric test. “N” represents the number of animals/group. The p values of statistically significant results are presented above the bars of the graph; non-significant (NS) data are also indicated.