Fig. 3.

Protein and mRNA expression in the small intestine of Ob/Ob mice. Five month old wild-type and Ob/Ob mice were euthanized, and the intestinal epithelium was removed, homogenized and processed for protein and mRNA expression: Panels A to G, as well as P and Q represent protein expression. Representative blots are displayed at the top of each graph. Densitometry was used to measure the intensity of each band, and these values, expressed in arbitrary units (A.U.), were normalized by Ponceau-S staining intensity. Panels H to O represent mRNA quantification as determined by RTq-PRC analyses. The number of animals analyzed for each group (N) is displayed above each bar. The results were analyzed using the Student's t-test and a non-parametric test was applied for NHE3 and MTTP protein content and for all the mRNAs evaluated. The remaining proteins were evaluated using a parametric test. The p values of statistically significant results are presented above the bars of the graph; non-significant (NS) data are also indicated. The nitrocellulose membrane was cut at the level of the target proteins using a standard molecular marker as guide. The full area of the selected blots is shown in supplementary data 2.