Fig. 5.

Protein expression in the small intestine of mice treated with H89. Mice fed a LFD for 12 weeks were treated with H89 or vehicle (5% DMSO), every 8 h, for 24 h. Mice were then euthanized, and the intestinal epithelium was removed, homogenized and processed for detecting the followings proteins: A: pCREB, B: GLUT2, C: PEPT1, D: NHE3, E: FAT-CD36, F: NPC1L1, G: MTTP and H: L-FABP. Representative blots are displayed at the top of each graph. The number of animals analyzed for each group (N) is also displayed above each bar. Densitometry was used to measure the intensity of each band, and these values, expressed in arbitrary units (A.U.), were normalized by Ponceau-S staining intensity. The results were analyzed using the Student's t-test. PEPT1, MTTP and L-FABP were subjected to the non-parametric test, and all the other proteins were evaluated using the parametric test. The p values of statistically significant results are presented above the bars of the graph; non-significant (NS) data are also indicated. The nitrocellulose membrane was cut at the level of the target proteins using a standard molecular marker as guide. The full area of the selected blots is shown in supplementary data 2.