Fig. 2.

Compound panel showing immunohistochemical findings on samples immunolabelled with antibody against collagen type I. The staining pattern for this protein show clearly detectable fluorescence in the periodontal ligament from control subjects (A). On the tension side, after 1 day of treatment, it was gradually increased (B); at 7 days (C) and severely at 14 days (D), the collagen type I staining pattern was reduced, whereas the fluorescence pattern was notably increased at 21 (E) and 30 days (E). On the pressure side, the staining pattern for collagen type I slightly increased after 1 (G) and 7 days (H) of treatment, whereas at 14 (I), 21 (J), and 30 days (K) of treatment, it notably increased. The blue fluorescence represents nuclear-staining DAPI confirming the presence of the cells.