Fig. 5.

Compound panel showing immunohistochemical findings on samples immunolabelled with antibody against VEGF. The staining pattern for this protein shows a clearly detectable fluorescence in the periodontal ligament from control subjects (A). Results on the tension side showed a strong fluorescence pattern at day 1 (B) that decreased from 7 (C) to 14 days (D) and increased from 21 (E) to 30 (F) days of treatment. On the pressure side, a weak fluorescence pattern is demonstrated at day 1 (F), 7 (G), and 14 (H) of treatment. Results after 21 (I) and 30 (J) days showed an increase in VEGF staining pattern, which was uniformly distributed along the tissue. The blue fluorescence represents nuclear-staining DAPI confirming the presence of the cells. The double-localization reaction between VEGF (green channel in L) and CD31 (red channel in L), performed at 30 days on the tension side, shows yellow fluorescence due to overlap of CD31 on VEGF, demonstrating a co-localization between the two proteins.