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. 2019 Oct 4;28:101337. doi: 10.1016/j.redox.2019.101337

Fig. 6.

Fig. 6

MNPQ-mediated changes in the levels of HP-induced senescent cells (A) and the levels of selected proteins involved in cell signaling, proinflammatory responses, autophagy and glucose uptake (B, C). (A) Senescence-associated β-galactosidase activity. Representative microphotographs are shown. Scale bars 100 μm, objective 20x. (B, C) Western blot analysis of the levels of AMPK, phospho-AMPK, LC3BII, IFN-β, IL-8, AKT, phospho-AKT and GLUT1. For evaluation of LC3BII levels, cells were also incubated with 50 μg/ml chloroquine for 6 h. Data were normalized to β-actin. Chloroquine-treated samples are denoted with an asterisk. The levels of phospho-AMPK and phospho-AKT are also presented as a ratio of phospho-AMPK to AMPK and phospho-AKT to AKT, respectively. IL-8 and IFN-β levels in supernatants were calculated per 10000 cells. Bars indicate SD, n = 3, ***p < 0.001, **p < 0.01, *p < 0.05 compared to CTRL, ###p < 0.001, ##p < 0.01, #p < 0.05 compared to HP-treated control (ANOVA and Dunnett's a posteriori test). CTRL, control conditions; Q, quercetin; MNP, magnetite nanoparticles; MNPQ, quercetin surface functionalized magnetite nanoparticles.