Fig. 7.

MNPQ-mediated effects on selected parameters of extracellular acidification rate (ECAR, Glycolysis Stress Test) (A) and oxygen consumption rate (OCR, Cell Mito Stress Test) (B). (A) Real-time measurements of glycolysis as ECAR parameters (from left to right): glycolysis after the addition of glucose, glycolytic capacity after the addition of oligomycin and glycolytic reserve after the addition of 2-DG. (B) Real-time measurements of mitochondrial oxidative phosphorylation (OXPHOS) as OCR parameters: basal respiration, ATP production and proton leak after the addition of oligomycin, maximal respiration after the addition of the uncoupler FCCP and spare respiratory capacity after the addition of rotenone and antimycin A. ECAR and OCR were measured using an extracellular flux analyzer (Seahorse XFp Analyzer). Bars indicate SD, n = 3, ***p < 0.001, **p < 0.01 compared to CTRL, ^##p < 0.01, ^#p < 0.05 compared to HP-treated control (ANOVA and Dunnett's a posteriori test). CTRL, control conditions; Q, quercetin; MNP, magnetite nanoparticles; MNPQ, quercetin surface functionalized magnetite nanoparticles.