Subject Area:	Biochemistry, Genetics and Molecular Biology
More specific subject area:	Analytical Biochemistry, Enzymology
Method name:	Dissociation of ACAT1 subunits by non-ionic detergents
Name and reference of original method:	B. Neumann, C.C.Y. Chang, T.Y. Chang, Triton X-100 or octyl glucoside inactivates acyl-CoA:cholesterol acyltransferase 1 by dissociating it from a two-fold dimer to a two-fold monomer, Archives of biochemistry and biophysics 671 (2019) 103–110.
	C. Yu, J. Chen, S. Lin, J. Liu, C.C. Chang, T.Y. Chang, Human acyl-CoA:cholesterol acyltransferase-1 is a homotetrameric enzyme in intact cells and in vitro, The Journal of biological chemistry 274(51) (1999) 36139–45.
	C.C. Chang, C.Y. Lee, E.T. Chang, J.C. Cruz, M.C. Levesque, T.Y. Chang, Recombinant acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) purified to essential homogeneity utilizes cholesterol in mixed micelles or in vesicles in a highly cooperative manner, The Journal of biological chemistry 273(52) (1998) 35132–41.
Resource availability:	[3H]oleoyl-CoA, water bath, Organomation N-EVAP Nitrogen Evaporator, Uniplate Analtech Silica gel HL, TLC developing tank, Ecoscint O, scintillation counter, peroxide-free Triton X-100, Beckman L8-70 M ultracentrifuge, SW 60 Ti rotor, Thermo Fisher Scientific HisPur Ni-NTA resin, Corning Costar Spin-X 0.22 μm centrifuge tube filters, USA Scientific 1.5 ml low adhesion microcentrifuge tubes, New Brunswick Scientific Gyrotory Shaker Model G2, Thermo Scientific GelCode Blue Stain Reagent, Pall Corporation 10 K Microsep Advance Centrifugal Device with Omega Membrane