Effects of long term adipose LCN2 overexpression. A, Study plan for long-term adipose LCN2 overexpression in female C57BL/6J wildtype (WT). B, Fat mass, lean mass, and total mass before and after 8-weeks of HF/HS diet challenge. C, Cumulative food consumption between GFP and LCN2 groups. D, Glucose-tolerance test and E, Insulin-tolerance test and their respective AUC of adipose-specific GFP or LCN2 overexpressing females after 8- and 10- weeks of diet challenge. Data are presented as mean ± SEM (n = 5 animals per group). P values were calculated by B, 3-factor ANOVA corrected by post-hoc “Holm-Sidak's” multiple comparisons test; D and E, Repeated measures 2-factor ANOVA corrected by post-hoc “Holm-Sidak's” multiple comparisons test; D and E, Unpaired Student's t test for AUC. *P < 0.05; **P < 0.01; ***P < 0.001.