Females with adipose LRP2 knockdown exhibit obesity and other metabolic disturbances. A, Fat mass and lean mass before and after 8-weeks of HF/HS diet challenge along with Lrp2 expression between the two groups. B, Cumulative food consumption between control and shLrp2 groups. C, Glucose-tolerance test and D, Insulin-tolerance test and their respective AUC of adipose-specific control or shLrp2 females after 8- and 10- weeks of diet challenge. Comparisons of E, plasma levels of insulin F, HOMA-IR and G, tissue weights and H, Electron flow assays of isolated gonadal adipose mitochondria between the two groups after HF/HS diet challenge. Data are presented as mean ± SEM (n = 6 animals per group for A – G; n = 3–4 mitochondria per group for H). P values were calculated by A, 3-factor ANOVA corrected by post-hoc “Holm-Sidak's” multiple comparisons test; A, Unpaired Student's t test for gene expression; C and D, Repeated measures 2-factor ANOVA corrected by post-hoc “Holm-Sidak's” multiple comparisons test; C – F, Unpaired Student's t test for AUC and insulin panel; G and H, 2-factor ANOVA corrected by post-hoc “Holm-Sidak's” multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001.