Fig. S1.

Effects of LDL treatment and siRNA-mediated LDLr knock-down on survival of adult hippocampal precursor cells in vitro. Precursor cells isolated from C57BL/6J mice were propagated as adherent monolayers and treated with: (A–C) human plasma LDL (0-200 μg/mL); (D–F) transfected with a pool of siRNAs targeting the LDLR gene or a pool of non-targeting siRNAs as a control; (G–I) or 27-HC (0–10 μM). (A, D, G) 24 h after each treatment, cell viability was measured by the resazurin assay. Hoechst staining allowed morphological analysis of apoptosis, as integrated density and average nucleus size after exposure to LDL (B–C) LDLR knock-down (E–F) and 27-HC treatment (H–I). Data are presented as means ± SEM. N = 3 per group. *p < 0.05, t- test (D–F), one-way ANOVA followed by Dunnett’s post hoc test (A–C, G–I).