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. 2019 Sep 11;30:1–15. doi: 10.1016/j.molmet.2019.09.002

Figure 4.

Figure 4

Effect of LDL treatment and LDLr siRNA transfection on lipid internalization and storage. (A) Representative images of precursor cells stained with LD540 to visualise lipid droplets (green; white arrows). Cell nuclei were counterstained with Hoechst (blue). (B–C) Quantification of lipid droplets per field in LDL (100 μg/mL) treated (B) and siRNA transfected cells (C). (D) Representative images of internalized fluorescent LDL. LDL-Bodipy conjugate (green) was added 60 min before cell fixation, while the second LDL pulse, LDL-DiI (orange), was added 20 min before cell fixation. NT: non-target control. Scale bars = 20 μm. Data are presented as mean ± SEM. N = 3 per group. *p < 0.05, t-test.