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. 2019 Sep 27;18(5):4137–4143. doi: 10.3892/etm.2019.8064

Figure 5.

Figure 5.

Effect of salidroside on TNF-α-induced MAPK and NF-κB activation in CMECs. (A) Western blot analysis of the phosphorylation of p38, Jnk, Erk and total p38, Jnk and Erk in CMECs treated with 10 ng/ml TNF-α for 15 min, after treatment with the indicated concentrations of salidroside for 12 h. (B) Western blot analysis of the phosphorylation of NF-κB p65, IκBα and total NF-κB p65 and IκBα in CMECs treated with 10 ng/ml TNF-α for 15 min, after treatment with the indicated concentrations of salidroside for 12 h. (C) CMECs were pre-incubated with 100 µM salidroside for 12 h and TNF-α (10 ng/ml) for 30 min. NF-κB p65 (green) was detected by immunofluorescence using an anti-NF-κB p65 antibody, and the nuclei were stained with DAPI (magnification, ×400). The values are shown as the mean ± standard deviation from three independent experiments. ##P<0.01 vs. respective control group; *P<0.05 and **P<0.01 vs. respective TNF-α-only group. TNF-α, tumor necrosis factor α; CMECs, cardiac microvascular endothelial cells; IκBα, inhibitor of NF-κB; p, phosphorylated; t, total.