VEGF, TGF-β1, TNF-α and IL-6 in each group were determined after treatment via western blotting. (A) The expression of the four proteins were determined using western blot analysis. (B) The expression of VEGF in the US treatment group was significantly increased on 14th and 21st day compared with the control group. (C) The expression of TGF-β1 in the US treatment group was significantly increased on 14th and 21st day compared with the control group. (D) The expression of TNF-α in the US treatment group were gradually reduced, with the lowest expression level being exhibited on the 21st day as treatment time was prolonged. (E) The expression of IL-6 in the US treatment group were gradually reduced, with the lowest expression level being exhibited on the 21st day. *P<0.05 and **P<0.01 as indicated; #P<0.05 as indicated. VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; US, ultrasound.