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. Author manuscript; available in PMC: 2020 Nov 7.
Published in final edited form as: J Mater Chem B. 2019 Sep 25;7(41):6449–6457. doi: 10.1039/c9tb01918a

Fig. 2.

Fig. 2

Micelle binding to HA microcrystals. A) Confocal images show HA PAMs (green, 5 μM) bind to HA microcrystals (brightfield) to a greater extent than NT micelles which exhibited minimal binding. Scale bar 100 μm. B) Quantification of micelle binding to HA microcrystals at 5 and 10 μM. **** indicates p≤0.0001. C) Competition assay shows specificity of HABP binding to HA. HA PAM bound 1.8-fold greater to HA microcrystals preincubated with scrambled HABPs (30.5 ± 4.2%) vs. HABPs (16.3 ± 3.6%, **** p≤0.0001, N=6) D) Binding isotherm of HA PAM on HA microcrystals show a KD of 6.25 ± 1.21 μM (N=3).