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. Author manuscript; available in PMC: 2020 Oct 23.
Published in final edited form as: J Am Chem Soc. 2019 Oct 15;141(42):16790–16801. doi: 10.1021/jacs.9b07396

Figure 2.

Figure 2.

MALDI-TOF mass spectra showing the [M+H]+ of (a) His6-OlvA co-expressed with OlvB and OlvC [His6-OlvA(BC)], (b) full-length OlvA(BCSA) after nickel affinity purification, and (c) after incubation at 37 °C for 18 h. Samples were reacted with N-ethylmaleimide to alkylate incompletely cyclized peptides and retain only the completely modified peptides in the 7100–7200 m/z observation range. (d) RP-HPLC profile of OlvA(BC)GluC, OlvA(BCSA)GluC, and co-injection of OlvA(BC)GluC and OlvA(BCSA)GluC. (e) ESI tandem mass spectrum of OlvA(BCSA)GluC.