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. Author manuscript; available in PMC: 2020 Oct 23.
Published in final edited form as: J Am Chem Soc. 2019 Oct 15;141(42):16790–16801. doi: 10.1021/jacs.9b07396

Figure 7.

Figure 7.

ESI-QTOF mass spectra (+2 charge state) of OlvA(BC)GluC from the 18O-labeling experiments; (a) OlvA(BC)GluC substrate incubated in buffer made in H218O, and (b) 50 μM OlvA(BC)GluC reacted with 10 μM OlvSA and 1 mM SAM in 50 mM Hepes made in H218O, pH 7. The spectrum in panel b is from a mixture of Asp and isoAsp containing peptides as explained in the text.