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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Sep 19;39(11):2261–2272. doi: 10.1161/ATVBAHA.119.313056

Figure 2. Myeloid deficiency of Plpp3 does not alter atherosclerosis.

Figure 2.

A) Plpp3 gene expression (fold change; mean ± SEM) in bone marrow-derived macrophages (BMDMs) from fl/fl and LysM-Δ mice (n = 3 – 5 per group) treated with human LDL (50 μg/mL), human oxLDL (50 μg/mL) or vehicle for 18 hours. Two-way ANOVA with Holm-Sidak pairwise multiple comparisons test was used to determine differences between treatment groups and genotype. B) Gene expression was profiled in BMDMs from fl/fl or LysM-Δ mice (n= 3 – 5/group) exposed to vehicle or human oxLDL. Two-way ANOVA with Holm-Sidak pairwise multiple comparisons test was used to analyze effects of genotype (G) and ox-LDL as an environmental stimulus for foam cell formation (E). C) Plasma cholesterol (mg/dL) in chimeric Ldlr−/− mice injected with bone marrow cells harvested from fl/fl (closed circles) or LysM-Δ (open circles) mice fed western diet for 12 weeks beginning 4 weeks after irradiation and reconstitution (n=10/group). D) Plasma LPA species quantification from fl/fl (black bars) and LysM-Δ (white bars) mice on the Ldlr−/− background after 12 weeks on Western diet (n=10/group). E) En face analysis of atherosclerosis (% of arch area) in Western-diet fed, chimeric Ldlr−/− mice with fl/fl (closed circles) or LysM-Δ (open circles) bone marrow cells (n=10/group). F) Oil red-O staining of aortic sinus lesions reported as % area of aortic sinus (n=10/group). *P<0.05