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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Sep 19;39(11):2261–2272. doi: 10.1161/ATVBAHA.119.313056

Figure 3. SMC deficiency of Plpp3 accelerates atherosclerosis.

Figure 3.

A) Plpp3 expression (fold change; mean ± SEM) from SMC isolated from fl/fl (dark) and SM22-Δ (open) mice on the Ldlr−/− background (n=3/group). B) At 6 – 8 weeks of age, fl/fl (dark circles) and SM22-Δ (open circles) mice on the Ldlr−/− background were placed on Western diet for 12 weeks and total plasma cholesterol (mg/dL) measured (n=7/group). C) Plasma LPA species quantification from fl/fl (black bars) and Sm22-Δ (white bars) mice on the Ldlr−/− background after 12 weeks on Western diet (n=9–11/group). D) En face analysis of atherosclerosis (% of arch area) (n=7–9/group). E) Oil red-O staining of aortic sinus lesions reported as % area of aortic sinus (n=5–6/group). F) Left Panel: Immunohistological quantification of CD68 from sections of aortic roots from fl/fl and MX1-Δ mice on the Ldlr−/− background after 12 weeks on Western diet (n=5–6/group). Right Panel: Representative images of CD68 staining in the aortic sinus. G) Plpp3 and Il-6 expression (fold change; mean ± SEM) in proximal aortas from fl/fl and SM22-Δ (n=5/group). H) Total LPA content (pmol/mg) in proximal aortas of fl/fl and SM22-Δ (n=7–9/group).