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. Author manuscript; available in PMC: 2019 Oct 24.
Published in final edited form as: Nutr Cancer. 2014 Jan 21;66(2):295–307. doi: 10.1080/01635581.2014.868911

Figure 5. Effect of MSeA on mTOR pathway signaling and autophagy in PANC-1 cells.

Figure 5.

A. Western blot detection of MSeA-induced changes of proteins or/and their phosphorylation of mTOR and its downstream targets and autophagy marker LC-3. B. Impact of inhibiting autophagy by knocking down Beclin-1 on MSeA-induced caspase-mediated apoptosis in PANC-1 cells after 48 h. PANC-1 cells were transiently transfected with siRNA for Beclin1. Protein lysates were probed with the antibodies against Beclin 1, LC3-II, and c-PARP. β-actin served as loading control. C. The enzymatic activities of caspase-3 and 6. Cells were treated with MSeA for 48 h. Results were plotted as mean ± SD from three independent experiments. **p<0.01 when compared with control.