| Antibodies |
| 5-Hydroxymethylcytosine (5hmC) antibody |
Active Motif |
Cat#39769; RRID: AB_10013602 |
| 5-Methylcytosine (5mC) antibody (mAb) |
Active Motif |
Cat#39649; RRID: AB_2687950 |
| Anti-ALKBH5 antibody |
Abcam |
Cat#ab195377; RRID: AB_1267666 |
| Anti-ASB2 antibody |
Abcam |
Cat#ab13710; RRID: AB_300589 |
| Anti-CEBP Alpha antibody |
Abcam |
Cat#ab15048; RRID: AB_2077890 |
| Anti-c-MYC antibody [Y69] |
Abcam |
Cat#ab32072; RRID: AB_731658 |
| Anti-FTO antibody [EPR6895] |
Abcam |
Cat#ab124892; RRID: AB_10972698 |
| Anti-RARA antibody |
BioLegend |
Cat#616801; RRID: AB_2253600 |
| Anti-Histone H3 |
Proteintech |
Cat#17168–1-AP; RRID: AB_2716755 |
| Anti-Histone H4 |
Cell Signaling Technology |
Cat#13919S |
| Anti-H3K4me1 |
Abcam |
Cat#ab176877; RRID: AB_2637011 |
| Anti-H3K4me2 |
Active Motif |
Cat#39914; RRID: AB_2614976 |
| Anti-H3K4me3 |
Cell Signaling Technology |
Cat#9751T; RRID: AB_2616028 |
| Anti-H3K9me2 |
Abcam |
Cat#ab1220; RRID: AB_449854 |
| Anti-H3K9me3 |
Cell Signaling Technology |
Cat#13969T |
| Anti-H3K27me2 |
Abcam |
Cat#ab24684; RRID: AB_448222 |
| Anti-H3K27me3 |
Active Motif |
Cat#39157; RRID: AB_2636821 |
| Anti-H3K36me2 |
Active Motif |
Cat#39892; RRID: AB_2793383 |
| Anti-H3K36me3 |
Cell Signaling Technology |
Cat#4909T; RRID: AB_1950412 |
| Anti-H3K79me1 |
Active Motif |
Cat#39922; RRID: AB_2793394 |
| Anti-H3K79me2 |
Active Motif |
Cat#39924; RRID: AB_2793395 |
| Anti-H3K79me3 |
Abcam |
Cat#ab2621; RRID: AB_303215 |
| Anti-H3R17me2a |
Active Motif |
Cat#39710; RRID: AB_2793315 |
| Anti-H4K20me1 |
Abcam |
Cat#ab177188 |
| Anti-H4K20me2 |
Active Motif |
Cat#39540; RRID: AB_2793248 |
| Anti-H4K20me3 |
Active Motif |
Cat#39672; RRID: AB_2650526 |
| Donkey anti-rabbit IgG-HRP |
Santa Cruz |
Cat#sc-2077; RRID: AB_631745 |
| GAPDH antibody (0411) |
Santa Cruz |
Cat#sc-47724; RRID: AB_627678 |
| Goat anti-mouse IgG-HRP |
Santa Cruz |
Cat#sc-2055; RRID: AB_631738 |
| m6A (N6-methyladenosine) antibody |
Synaptic Systems |
Cat#202003; RRID: AB_2279214 |
| Biological Samples |
| Leukemic patients sample sections |
Cincinnati Children’s Hospital Medical Center |
N/A |
| Chemical, Peptides, and Recombinant protein |
| ATRA |
Sigma-Aldrich |
Cat#223018 |
| Ciclopirox |
Topscience |
Cat#T1482 |
| FBS |
GIBCO |
Cat#10099–141 |
| Giemsa Stain |
EMD chemicals |
Cat#620G-75 |
| IOX-1 |
Topscience |
Cat#T6545 |
| JIB-04 |
MCE |
Cat#HY-13953 |
| May-Grunwald Stain |
Sigma-Aldrich |
Cat#MG-500 |
| Polybrene |
Sigma-Aldrich |
Cat#H9268 |
| Pronase from Streptomyces griseus |
Roche |
Cat#10165921001 |
| Puromycin dihydrochloride |
Sigma-Aldrich |
Cat#P8833 |
| Recombinant Human Flt3-Ligand |
PeproTech |
Cat#300–19 |
| Recombinant Human IL-3 |
PeproTech |
Cat#200–03 |
| Recombinant Human IL-6 |
PeproTech |
Cat#200–06 |
| Recombinant Human SCF |
PeproTech |
Cat#300–07 |
| Recombinant Human TPO |
PeproTech |
Cat#300–18 |
| Recombinant Mouse IL-3 |
PeproTech |
Cat#213–03 |
| Recombinant Mouse SCF |
PeproTech |
Cat#250–03 |
| RppH |
NEB |
Cat#M0356S |
| TET1 |
Active Motif |
Cat#31417 |
| Critical Commercial Assays |
| CellTiter 96 Non-Radioactive Cell Proliferation Assay (MTT) |
Promega |
Cat#G4100 |
| COX Fluorescent Inhibitor Screening Assay Kit |
Cayman |
Cat#700100 |
| Epigenetic Screening |
Shanghai ChemPartner Co., Ltd |
CPB-P16–20033 |
| FITC Annexin V Apoptosis Detection Kit I |
BD Biosciences |
Cat#556547 |
| IC50Profiler™ |
Eurofins Pharma Discovery Services UK Limited |
UK022–0004210 |
| KinaseProfiler™ |
Eurofins Pharma Discovery Services UK Limited |
UK022–0004141 |
| miRNeasy Mini Kit |
QIAGEN |
Cat#217004 |
| PE Annexin V Apoptosis Detection Kit I |
BD Biosciences |
Cat#559763 |
| PolyATract mRNA isolation System IV |
Promega |
Cat#Z5310 |
| ProteaseProfiler™ |
Eurofins Panlabs Discovery Services Taiwan Limited |
TW04–0002669 |
| RiboMinus Transcriptome Isolation Kit |
Invitrogen |
Cat#A1083708 |
| Deposit Data |
| m6A-seq |
This paper |
GEO: GSE103496
|
| RNA-seq |
This paper |
GEO: GSE103494, GSE103495
|
| Structural complex of FTO/FB23 |
This paper |
PDB: 6AKW |
| X-ray structure of FB23-2 |
This paper |
CCDC: 1558332 |
| Experimental Models: Cell line |
| 293T |
ATCC |
CRL-3216 |
| FLT3ITD/NPM1 |
Isolated from BM of AML mouse induced by FLT3ITD/NPM1 |
N/A |
| MA9 |
Isolated from BM of AML mouse induced by MA9 oncogene |
N/A |
| MA9.3ITD |
A gift from James C. Mulloy lab |
N/A |
| MA9.3RAS |
A gift from James C. Mulloy lab |
N/A |
| ML-2 |
DSMZ |
ACC-15 |
| MONOMAC6 |
DSMZ |
ACC-124 |
| MV4–11 |
ATCC |
CRL-9591 |
| NB4 |
DSMZ |
ACC-207 |
| U937 |
ATCC |
CRL-1593.2 |
| Experimental Models: Organisms/Strains |
| BALB/c mouse |
Shanghai SLAC Laboratory Animals Co. Ltd. |
N/A |
| NSGS mouse |
The Jackson Laboratory |
Stock#013062 |
| SD rat |
Shanghai SLAC Laboratory Animals Co. Ltd. |
N/A |
| Oligonucleotides |
| 5′-AUUGUCA(m6A)CAGCAGC-3′ |
TAKARA, China |
N/A |
| 5’-CAGTAACTGTGGTC(5mC)GGTAACTGACTTGCA-3’ |
Sangon Biotech, China |
N/A |
| See Table S9 for primers |
Generay, China |
N/A |
| Recombinant DNA |
| pLKO.1-shFTO |
This paper |
N/A |
| pLKO.1-shNS |
This paper |
N/A |
| pWSLV-cas02-FTO-KO |
This paper |
N/A |
