Antibodies |
5-Hydroxymethylcytosine (5hmC) antibody |
Active Motif |
Cat#39769; RRID: AB_10013602 |
5-Methylcytosine (5mC) antibody (mAb) |
Active Motif |
Cat#39649; RRID: AB_2687950 |
Anti-ALKBH5 antibody |
Abcam |
Cat#ab195377; RRID: AB_1267666 |
Anti-ASB2 antibody |
Abcam |
Cat#ab13710; RRID: AB_300589 |
Anti-CEBP Alpha antibody |
Abcam |
Cat#ab15048; RRID: AB_2077890 |
Anti-c-MYC antibody [Y69] |
Abcam |
Cat#ab32072; RRID: AB_731658 |
Anti-FTO antibody [EPR6895] |
Abcam |
Cat#ab124892; RRID: AB_10972698 |
Anti-RARA antibody |
BioLegend |
Cat#616801; RRID: AB_2253600 |
Anti-Histone H3 |
Proteintech |
Cat#17168–1-AP; RRID: AB_2716755 |
Anti-Histone H4 |
Cell Signaling Technology |
Cat#13919S |
Anti-H3K4me1 |
Abcam |
Cat#ab176877; RRID: AB_2637011 |
Anti-H3K4me2 |
Active Motif |
Cat#39914; RRID: AB_2614976 |
Anti-H3K4me3 |
Cell Signaling Technology |
Cat#9751T; RRID: AB_2616028 |
Anti-H3K9me2 |
Abcam |
Cat#ab1220; RRID: AB_449854 |
Anti-H3K9me3 |
Cell Signaling Technology |
Cat#13969T |
Anti-H3K27me2 |
Abcam |
Cat#ab24684; RRID: AB_448222 |
Anti-H3K27me3 |
Active Motif |
Cat#39157; RRID: AB_2636821 |
Anti-H3K36me2 |
Active Motif |
Cat#39892; RRID: AB_2793383 |
Anti-H3K36me3 |
Cell Signaling Technology |
Cat#4909T; RRID: AB_1950412 |
Anti-H3K79me1 |
Active Motif |
Cat#39922; RRID: AB_2793394 |
Anti-H3K79me2 |
Active Motif |
Cat#39924; RRID: AB_2793395 |
Anti-H3K79me3 |
Abcam |
Cat#ab2621; RRID: AB_303215 |
Anti-H3R17me2a |
Active Motif |
Cat#39710; RRID: AB_2793315 |
Anti-H4K20me1 |
Abcam |
Cat#ab177188 |
Anti-H4K20me2 |
Active Motif |
Cat#39540; RRID: AB_2793248 |
Anti-H4K20me3 |
Active Motif |
Cat#39672; RRID: AB_2650526 |
Donkey anti-rabbit IgG-HRP |
Santa Cruz |
Cat#sc-2077; RRID: AB_631745 |
GAPDH antibody (0411) |
Santa Cruz |
Cat#sc-47724; RRID: AB_627678 |
Goat anti-mouse IgG-HRP |
Santa Cruz |
Cat#sc-2055; RRID: AB_631738 |
m6A (N6-methyladenosine) antibody |
Synaptic Systems |
Cat#202003; RRID: AB_2279214 |
Biological Samples |
Leukemic patients sample sections |
Cincinnati Children’s Hospital Medical Center |
N/A |
Chemical, Peptides, and Recombinant protein |
ATRA |
Sigma-Aldrich |
Cat#223018 |
Ciclopirox |
Topscience |
Cat#T1482 |
FBS |
GIBCO |
Cat#10099–141 |
Giemsa Stain |
EMD chemicals |
Cat#620G-75 |
IOX-1 |
Topscience |
Cat#T6545 |
JIB-04 |
MCE |
Cat#HY-13953 |
May-Grunwald Stain |
Sigma-Aldrich |
Cat#MG-500 |
Polybrene |
Sigma-Aldrich |
Cat#H9268 |
Pronase from Streptomyces griseus |
Roche |
Cat#10165921001 |
Puromycin dihydrochloride |
Sigma-Aldrich |
Cat#P8833 |
Recombinant Human Flt3-Ligand |
PeproTech |
Cat#300–19 |
Recombinant Human IL-3 |
PeproTech |
Cat#200–03 |
Recombinant Human IL-6 |
PeproTech |
Cat#200–06 |
Recombinant Human SCF |
PeproTech |
Cat#300–07 |
Recombinant Human TPO |
PeproTech |
Cat#300–18 |
Recombinant Mouse IL-3 |
PeproTech |
Cat#213–03 |
Recombinant Mouse SCF |
PeproTech |
Cat#250–03 |
RppH |
NEB |
Cat#M0356S |
TET1 |
Active Motif |
Cat#31417 |
Critical Commercial Assays |
CellTiter 96 Non-Radioactive Cell Proliferation Assay (MTT) |
Promega |
Cat#G4100 |
COX Fluorescent Inhibitor Screening Assay Kit |
Cayman |
Cat#700100 |
Epigenetic Screening |
Shanghai ChemPartner Co., Ltd |
CPB-P16–20033 |
FITC Annexin V Apoptosis Detection Kit I |
BD Biosciences |
Cat#556547 |
IC50Profiler™ |
Eurofins Pharma Discovery Services UK Limited |
UK022–0004210 |
KinaseProfiler™ |
Eurofins Pharma Discovery Services UK Limited |
UK022–0004141 |
miRNeasy Mini Kit |
QIAGEN |
Cat#217004 |
PE Annexin V Apoptosis Detection Kit I |
BD Biosciences |
Cat#559763 |
PolyATract mRNA isolation System IV |
Promega |
Cat#Z5310 |
ProteaseProfiler™ |
Eurofins Panlabs Discovery Services Taiwan Limited |
TW04–0002669 |
RiboMinus Transcriptome Isolation Kit |
Invitrogen |
Cat#A1083708 |
Deposit Data |
m6A-seq |
This paper |
GEO: GSE103496
|
RNA-seq |
This paper |
GEO: GSE103494, GSE103495
|
Structural complex of FTO/FB23 |
This paper |
PDB: 6AKW |
X-ray structure of FB23-2 |
This paper |
CCDC: 1558332 |
Experimental Models: Cell line |
293T |
ATCC |
CRL-3216 |
FLT3ITD/NPM1 |
Isolated from BM of AML mouse induced by FLT3ITD/NPM1 |
N/A |
MA9 |
Isolated from BM of AML mouse induced by MA9 oncogene |
N/A |
MA9.3ITD |
A gift from James C. Mulloy lab |
N/A |
MA9.3RAS |
A gift from James C. Mulloy lab |
N/A |
ML-2 |
DSMZ |
ACC-15 |
MONOMAC6 |
DSMZ |
ACC-124 |
MV4–11 |
ATCC |
CRL-9591 |
NB4 |
DSMZ |
ACC-207 |
U937 |
ATCC |
CRL-1593.2 |
Experimental Models: Organisms/Strains |
BALB/c mouse |
Shanghai SLAC Laboratory Animals Co. Ltd. |
N/A |
NSGS mouse |
The Jackson Laboratory |
Stock#013062 |
SD rat |
Shanghai SLAC Laboratory Animals Co. Ltd. |
N/A |
Oligonucleotides |
5′-AUUGUCA(m6A)CAGCAGC-3′ |
TAKARA, China |
N/A |
5’-CAGTAACTGTGGTC(5mC)GGTAACTGACTTGCA-3’ |
Sangon Biotech, China |
N/A |
See Table S9 for primers |
Generay, China |
N/A |
Recombinant DNA |
pLKO.1-shFTO |
This paper |
N/A |
pLKO.1-shNS |
This paper |
N/A |
pWSLV-cas02-FTO-KO |
This paper |
N/A |