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. Author manuscript; available in PMC: 2019 Oct 24.
Published in final edited form as: Cell Rep. 2019 Mar 5;26(10):2636–2650.e5. doi: 10.1016/j.celrep.2019.02.004

Figure 3. Hyperactivation of YAP1 Upregulates Expression of the Putative HPV Receptor Molecules In vitro and In vivo.

Figure 3.

(A) RT-PCR analyses showing relative expression levels of the putative HPV receptors, including ITGA6, SDC1, and EGFR, in hCerEC-MX and hCerEC-YAPS127A cells. Each bar represents mean ± SEM (n = 4). *p < 0.05, **p < 0.01, and ***p < 0.001.

(B) RT-PCR analyses showing expression of ITGA6, SDC1, and EGFR in SiHa-CTRL and SiHa-siYAP cells. Each bar represents mean ± SEM (n = 4). *p < 0.05, **p < 0.01, and ***p < 0.001.

(C) Representative images showing HPV16 PsV-derived GFP signal in hCerEC-MX, hCerEC-YAP, and hCerEC-YAPS127A cells with (siITGA6) or without (siControl) knockdown of ITGA6 protein. Scale bar: 100 μm.

(D) Representative images showing expressions of YAP1 and the well-studied putative HPV receptor molecule ITGA6, in cervical tissues derived from 10-month-old control (KRT-rtTA mice) and KRT14-YAPS127A transgenic mice. Protein expression was determined using peroxidase-based immunohistochemistry. The nuclei were counterstained with hematoxylin. Note upregulation of ITGA6 protein in the epithelium of KRT14-YAPS127A transgenic mice. Scale bar: 200 μm.