KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Ki167 | Abcam (Cambridge, MA) | ab15580 |
| SDC1 | Abcam (Cambridge, MA) | ab188861 |
| p40(deltaNp63) | Ventana Medical Systems, Inc. (Tucson, AZ) | 790–4950 |
| P53 | Santa Cruz Biotechnology Inc (Dallas, TX) | sc-126 |
| ISG15 | Santa Cruz Biotechnology Inc (Dallas, TX) | sc-166755 |
| YAP | Cell Signaling Technology Inc. (Danvers, MA) | #4912 |
| phospho-YAP (Ser127) | Cell Signaling Technology Inc. (Danvers, MA) | #4911 |
| Pan KRT | Cell Signaling Technology Inc. (Danvers, MA) | #4545 |
| EGFR | Cell Signaling Technology Inc. (Danvers, MA) | #4267 |
| ITGA6 | Cell Signaling Technology Inc. (Danvers, MA) | #3750 |
| TLR1 | Cell Signaling Technology Inc. (Danvers, MA) | #2209 |
| TLR2 | Cell Signaling Technology Inc. (Danvers, MA) | #1227 |
| MYD88 | Cell Signaling Technology Inc. (Danvers, MA) | #4283 |
| TRIF | Cell Signaling Technology Inc. (Danvers, MA) | #4596 |
| TBK1 | Cell Signaling Technology Inc. (Danvers, MA) | #3504 |
| NF-κB1 p105/p50 | Cell Signaling Technology Inc. (Danvers, MA) | #12540 |
| NF-κB2 p100/p52 | Cell Signaling Technology Inc. (Danvers, MA) | #3017 |
| JAK1 | Cell Signaling Technology Inc. (Danvers, MA) | #3344 |
| JAK2 | Cell Signaling Technology Inc. (Danvers, MA) | #3230 |
| STAT1 | Cell Signaling Technology Inc. (Danvers, MA) | #9172 |
| STAT2 | Cell Signaling Technology Inc. (Danvers, MA) | #72604 |
| IRF3 | Cell Signaling Technology Inc. (Danvers, MA) | #11904 |
| IRF9 | Cell Signaling Technology Inc. (Danvers, MA) | #76684 |
| phospho-STAT1 | Cell Signaling Technology Inc. (Danvers, MA) | #7649 |
| phospho-STAT2 | Cell Signaling Technology Inc. (Danvers, MA) | #88410 |
| β-actin | Sigma-Aldrich (St. Louis, MO) | #A5441 |
| Bacterial and Virus Strains | ||
| p16L1L2 | Addgene | #45291 |
| pCIneoEGFP | Addgene | #46949 |
| MXIV-neo | Jixing Dong Lab | N/A |
| YAP-neo | Jixing Dong Lab | N/A |
| YAPS127A-neo | Jixing Dong Lab | N/A |
| Biological Samples | ||
| The human cervical tissues microarray | US Biomax, Inc | BB10011 |
| Panomics, Inc. | CIN481, 482, & 483 | |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Interferon alpha 2b | R&D systems Inc. | #11105–1 |
| Critical Commercial Assays | ||
| RNAeasy micro kit | QIAGEN | #74004 |
| iScript Reverse Transcription Supermix for RT-qPCR | bio-rad | #1708841 |
| iTaq Universal SYBR® Green Supermix | bio-rad | #1725121 |
| VECTASTAIN® Elite® ABC HRP Kit (Peroxidase, Rabbit IgG) | VECTOR LABORATORIES | PK-6103 |
| Antigen Unmasking Solution, Citric Acid Based | VECTOR LABORATORIES | H-3300 |
| ImmPACT® DAB Peroxidase (HRP) Substrate | VECTOR LABORATORIES | SK-4105 |
| ImmPACT® NovaRED Peroxidase (HRP) Substrate | VECTOR LABORATORIES | SK-4805 |
| VECTASTAIN® ABC-AP Staining KIT (Alkaline Phosphatase, Rabbit IgG) | VECTOR LABORATORIES | AK-5001 |
| BLOXALL® Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution | VECTOR LABORATORIES | SP-6000 |
| VECTOR® Red Alkaline Phosphatase (Red AP) Substrate Kit | VECTOR LABORATORIES | SK-5100 |
| Experimental Models: Cell Lines | ||
| Primary hCerECs | ScienCell Research Laboratories | #7060 |
| Ect1/E6E7 cells | ATCC | CRL-2614 |
| SiHa | ATCC | HTB-35 |
| Experimental Models: Organisms/Strains | ||
| KRT14-rtTA Mice (FVB-Tg(KRT14-rtTA) F42Efu/J) | The Jackson Laboratory | #008099 |
| TRE-YAPS127A mice | Dr. Fernando Camargo’s lab | N/A |
| KRT14-E6 mice | Dr. Lambert’s laboratory (Herber et al., 1996; Song et al., 1999) | N/A |
| KRT14-E7 mice | Dr. Lambert’s laboratory (Herber et al., 1996; Song et al., 1999) | N/A |
| Oligonucleotides | ||
| Primers for qRT-PCR, see Table S1 | This Paper | N/A |
| Primers for genotyping, see Table S1 | This Paper | N/A |
| siRNA (smart pool) targeting YAP1 | Dharmacon | M-012200-00-0005 |
| siRNA (#1) targeting YAP1 | Dharmacon | D-012200-01-0002 |
| siRNA (#2) targeting YAP1 | Dharmacon | D-012200-02-0002 |
| siRNA (smart pool) targeting ITGA6 | Dharmacon | D-007214-01-0005 |
| siRNA (#1) targeting ITGA6 | Dharmacon | D-007214-01-0002 |
| siRNA (#2) targeting ITGA6 | Dharmacon | D-007214-02-0002 |