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. 2019 Oct 17;10:2421. doi: 10.3389/fimmu.2019.02421

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Copyright © 2019 He, Yang, Mu, Zhou, Chen, Wu, Wang, Yang, Li, Xu and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ot-WHP increased the numbers of neutrophils in wounds. (A) Images of wound sections stained with anti-mouse Ly6G. (B) Number of neutrophils per microscopic field. Each wound in BABL/c mice (n = 6) was treated with vehicle (PBS, 20 μl/wound/day), Ot-WHP (20 μl/wound/day, 200 μg/ml), or EGF (20 μl, 100 μg/ml, positive control), and wounds were taken at days 0.5 and 1 post injury. The biopsy of wounds including healed tissues (about 8 mm in diameter) were embedded in paraffin wax, sectioned and incubated with rat anti-mouse Ly6G antibody, number of neutrophils (Ly6G+ cells, indicated by arrows) per microscopic field in a 40 × objective were calculated by Image J from three random fields of each wound from 6 mice. ns, no significance, *p < 0.05.