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. 2019 Oct 17;10:2421. doi: 10.3389/fimmu.2019.02421

Figure 5.

Figure 5

Ot-WHP modestly promoted neutrophil phagocytosis and PMA-induced neutrophil extracellular trap formation. (A) Ot-WHP (25, 50, 100 μg/ml) modestly promoted neutrophil phagocytosis against S. aureus and E. coli. Neutrophils were pre-incubated with peptide or vehicle (PBS) for 1 h, and CFSE-labeled bacterial particles were added and incubated for 1.5 h. (B) Effect of Ot-WHP on NET formation. Neutrophils were incubated with vehicle (PBS), Ot-WHP (25, 50, 100 μg/ml), PMA (100 nM), or PMA (100 nM) + Ot-WHP (50 μg/ml) at 37°C for 4 h, respectively. Nuclei and NETs were stained with DAPI (blue) or anti-Cit-H3 (green), respectively. NETs were observed using a confocal microscope (×60, Nikon, Japan). The average of 3 independent experiments is shown. ns, no significance, *p < 0.05, **p < 0.01, ***p < 0.001.