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. 2019 Oct 17;10:2421. doi: 10.3389/fimmu.2019.02421

Figure 7.

Figure 7

Ot-WHP induced chemokine and cytokine production in mouse BMDMs and wound sites. (A) After treatment for 24 h, Ot-WHP (25, 50, 100 μg/ml) induced chemokine (CXCL1, CXCL2, CXCL3, and CCL2) and cytokine (TNF-α, IL-1β, IL-6, and TGF-β1) production in BMDMs in a dose-dependent manner. (B) Ot-WHP regulated chemokine (CXCL1 and CCL2) and cytokine (TNF-α) and transforming growth factor (TGF-β1) production in wound sites at indicated time points post injury. A same volume of vehicle (PBS) served as control. The biopsy of wound including healed tissues (about 8 mm in diameter) from each mouse (n = 6) was taken at days 0, 0.5, 1, 2, 4, and 6, and was ground into homogenate in 0.1 M PBS (containing 1 mM PMSF, 1 ml/g tissue) by a glass homogenizer. The levels of cytokine and chemokine in the culture medium of BMDMs and the supernatant of wound homogenates were quantified by ELISA. The average of 3 independent experiments is shown. ns, no significance, *p < 0.05, **p < 0.01, ***p < 0.001.