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. 2019 Jul 10;70(20):5643–5657. doi: 10.1093/jxb/erz323

Fig. 4.

Fig. 4.

Independent data validation by in situ hybridization. Expression in reproductive nucellus tissues of young flower buds was confirmed for Bostr.3279s0010 in B. divaricarpa ES517 (A, B), for Bostr.7867s1023 in B. stricta LTM (C–F), for Bostr.7867s1594 in B. divaricarpa ES517 (G, H) and in B. stricta LTM (I–K), and for Bostr.13129s0275 in B. stricta LTM (L–O). In situ hybridizations were performed with antisense probes (A, C–E, G, I, J, L–N) or with sense probes as controls (B, F, H, K, O). Arrows indicate the specifying megaspore mother cells (MMCs) or apomictic/aposporous initial cells (AICs), with exception of (E) where it indicates the functional megaspore. Scale bars are 50 µm.