Fig. 3.

NTRC and 2-Cys PRXs are present in the same fractions. Western blot analysis of fractions eluted with imidazole from GFP- (line #3-21-6) and NTRC- (line #5-24) expressing plants probed with the anti-2-Cys PRX (A) or the anti-NTRC antibody (B). As reference, leaf crude extracts (30 µg of protein) from WT and ntrc plants were loaded and, after blotting, the filter was excised in two parts at the molecular markers line to allow a more extended exposure of a part of the filter. The molecular mass in kDa of protein markers (M) is indicated on the left. end-NTRC, endogenous NTRC; tag-NTRC, tagged NTRC; agg, aggregated; mon, monomer; dim, dimer.