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. 2019 Jul 10;70(20):5895–5908. doi: 10.1093/jxb/erz327

Fig. 8.

Fig. 8.

Alternaria alternata-elicited NaEAS12 transcripts are largely dependent on NaERF2-like, and transient overexpression of NaERF2-like leads to transactivation of NaEAS12 promoter. (A) Mean (±SE) relative A. alternata-induced NaEAS12 transcripts as measured by real-time PCR in five replicate young leaves of EV and VIGS NaERF2-like plants at 2 dpi. Asterisks indicate level of significant difference between EV and VIGS leaves at 2 dpi (Student’s t-test: ***P<0.005). (B) Transcript abundance of NaERF2-like-2HA-eGFP and LUC in N. attenuata protoplasts co-expressing NaEAS12 promoter::LUC and 35S::2HA-eGFP or 35S::NaERF2-like-2HA-eGFP measured in three biological samples. Experiments were repeated twice with similar results. Asterisks indicate level of significant difference between control and NaERF2-like overexpressing protoplasts (Student’s t-test: *P<0.05; ***P<0.005).