Fig. 1.

Enhanced Botrytis immunity in the ABA hypersensitive era1-2 mutant. (A) Botrytis infection of Col-0, pp2c-quad, and era1-2. Droplets of Botrytis conidia suspensions (3 μl, 2×10⁶ spores ml^–1) were applied to 24-day-old fully expanded leaves. Photographs were taken at 3 days post infection (dpi). Scale bar=5 mm. (B) Quantitative data of lesion sizes in (A). The lesion diameters were measured with ImageJ. Combined results of four experiments (n=10 in each independent biological repeat) were analyzed in a linear mixed model with single-step P-value adjustment. Error bars represent the SE of means. Different letters above the bars indicate significant differences (P<0.05). (C) DNA of Botrytis on leaves at 3 dpi was quantified with real-time qPCR using the Arabidopsis actin gene as a control. Different letters indicate significant differences (two-tailed t-test, P<0.05). (D–G) Leaves were stained at 36 hours post infection (hpi) for cell death with trypan blue (D, F), and at 16 hpi for H₂O₂ accumulation with 3,3′-diaminobenzidine (E, G). The percentage stained area was used for comparisons between Col-0 and era1-2. Two biological repeats with seven leaves in each repeat were analyzed with a linear model. Different letters above the bars indicate significant differences (P<0.05). Scale bar=2 mm.