Fig. 2.

Enhanced cuticle permeability in the ABA hypersensitive era1-2 mutant. (A, B) The cuticle of era1-2 was more permeable than other mutants or the wild type Col-0 under toluidine blue staining. Leaves of 24-day-old plants were stained with 5 μl droplets of 0.05% toluidine blue solution for 2 h. The ABA-insensitive snrk2.236 was used as a positive control. (C) Quantification of the toluidine blue-stained areas. Combined results of three experiments (n=12 in each independent biological repeat) were analyzed in a linear mixed model with single-step P-value adjustment. Error bars represent the SE of means. Different letters above the bars indicate significant differences (P<0.05). (D) Leaves immersed in toluidine blue solution for 20 min. Bar=5 mm.