Fig. 6.

The Botrytis immunity of era1-2 was fully suppressed by bos1. (A) Typical lesion symptoms of era1-2 and bos1 era1-2. The arrows indicate Botrytis-infected lesions. Droplets of Botrytis conidia suspensions (3 μl, 2×10⁶ spores ml^–1) were applied to 24-day-old fully expanded leaves. Photographs were taken at 3 dpi. Scale bar=1 cm. (B) Quantitative lesion size data. The lesion diameters were measured with ImageJ. Combined results of three biological experiments (n=36 in total) were analyzed in a linear mixed model with single-step P-value adjustment. Error bars represent the SE of means. Different letters above the bars indicate significant differences (P<0.05). (C) Representative wound-induced cell death symptoms stained with trypan blue. Needle-punctured leaves were stained with trypan blue at 6 days post wounding to detect cell death. L, Length of cell death spread; P, puncture site. Scale bar=1 mm. (B) Quantitative spreading cell death data. The length of spread of cell death was measured as indicated in (C) around each wound four times in four directions (up, down, left, and right), and the mean value was used. Data of three repeats were analyzed in a linear mixed model with single-step P-value adjustment. Error bars represent the SE of means. Different letters above the bars indicate significant differences (P<0.05). (E) Spreading cell-death symptoms were enhanced in bos1 era1-2. Four-week-old in vitro-grown plants are shown. Once buds started opening, cell death initiated in the buds and then spread along the shoots, eventually causing the death of the whole plant. Right panel, close-up of the area indicated by the dashed box in the left panel. Arrows indicate dead buds. Scale bar=1 cm.