Fig 2. Follicular B lymphocytes were significantly depleted with APRIL/BLyS blockade, but newly formed B cells remained unchanged.

Flow cytometry was used to assess immature and mature B lymphocyte populations in spleen. Each graph shows number of cells per 100,000 lymphocytes. (A) Follicular B lymphocytes were defined as CD3^-CD21^intCD23⁺. (B) Newly formed B cells were identified as CD3^-CD21^-CD23^-. (C) Representative flow cytometry data of follicular and newly formed B cells by group. Number shown represents percentage of total cells in gate. *p<0.05, **p<0.008.