Fig 3. APRIL/BLyS blockade significantly depleted mature B and transitional 2 (T2) marginal zone B lymphocyte subsets, but preserved immature B lymphocytes.

Flow cytometry was used to assess immature and mature B lymphocyte populations in spleen. Each graph shows number of cells per 100,000 lymphocytes. (A) T1 B cells were defined as CD3^-CD21^-IgM⁺⁺. (B) T2 marginal zone B cells were identified as CD3^-CD21⁺⁺IgM⁺⁺. (C) Mature B cells were defined as CD3^-CD21⁺IgM⁺. *p<0.03, **p<0.004.