Table 5. Sensitivity and specificity of mNGS normalized reads for PCR target viruses.
PPV: positive predictive value, NPV, Negative predictive value.
Cut-off number of reads | |||
---|---|---|---|
0 | 15 | 50 | |
Sensitivity | 96% (23/24*) | 92% (22/24) | 83% (20/24) |
Specificity | 98% (1093/1120) | 100% (1118/1120) | 100% (1118/1120) |
PPV | 46% (23/50) | 92% (22/24) | 91% (20/22) |
NPV | 100% (1093/1094) | 100% (1118/1120) | 100% (1118/1122) |
*The sample with positive confirmatory OC43 PCR included.