Table 3. Proteins more abundant in R17.
| Category | Predicted Functions | Description (cn) 1 | P2 (n)3 | Score4 | Clone P178-13 C7 | Clone R17-12 C1 | Fold Change | ANOVA p Value | Accession Number |
|---|---|---|---|---|---|---|---|---|---|
| Cell adhesion | Cell adhesion Protease |
Immuno-dominant variable surface antigen-like [Trichomonas vaginalis G3] (1) | 3 (3) | 53,94 | 4,89E+04 | 9,28E+05 | 18,96 | 2,95E-04 | gi|12191712 |
| Cell adhesion | Cell adhesion Protease |
GP63-like [Trichomonas vaginalis G3] (2) | 2 (2) | 38,73 | 6,63E+04 | 5,16E+05 | 7,78 | 3,85E-07 | gi|121892295 |
| Cellular metabolism | Cell adhesion Cytoskeleton Protein metabolism |
Armadillo/beta-catenin-like repeat family protein [Trichomonas vaginalis G3] (3) | 2 (2) | 26,7 | 1,56E+04 | 7,89E+04 | 5,05 | 2,14E-08 | gi|121911734 |
| Cellular component | Cytoskeleton | alpha-tubulin [Trichonympha agilis] (4) | 6 (4) | 481,79 | 4,81E+04 | 2,01E+05 | 4,18 | 4,92E-03 | gi|496528628 |
| Cellular component | Cytoskeleton | alpha-tubulin 2, partial [Tritrichomonas foetus] (5) | 3 (1) | 115,83 | 5022,1 | 1,79E+04 | 3,57 | 9,13E-05 | gi|33465441 |
| Cellular component | Cytoskeleton Vesicle mediated transport |
Tctex-1 family protein [Trichomonas vaginalis G3] (6) | 4 (4) | 142,29 | 8039,24 | 2,53E+04 | 3,15 | 1,17E-06 | gi|121902586 |
| Cellular component | Cytoskeleton Rho GTPase binding Calmodulin binding |
Erythrocyte membrane-associated giant protein antigen 332 containing protein [Trichomonas vaginalis G3] (7) | 3 (3) | 58,8 | 1,48E+04 | 1,51E+05 | 10,23 | 7,82E-08 | gi|121905491 |
| Signal transduction | Small GTPase regulator Cytoskeletal protein |
RhoGAP domain containing protein [Trichomonas vaginalis G3] (8) | 2 (2) | 32,11 | 1,10E+05 | 5,12E+05 | 4,68 | 1,22E-05 | gi|121898182 |
| Signal transduction | Small GTPase regulator | ARF GAP-like zinc finger-containing protein [Trichomonas vaginalis G3] (9) | 2 (2) | 51,31 | 8180,38 | 5,29E+04 | 6,47 | 3,75E-06 | gi|121912557 |
| Signal transduction | Small GTPase regulator | RhoGEF domain containing protein [Trichomonas vaginalis G3] (10) | 2 (2) | 37,87 | 6003,78 | 3,60E+04 | 5,99 | 1,62E-05 | gi|121917299 |
| Signal transduction | Small GTPase regulator | Ras family protein [Trichomonas vaginalis G3] (11) | 4 (4) | 273,64 | 3,51E+04 | 1,33E+05 | 3,8 | 4,79E-08 | gi|121900431 |
| Cellular metabolism | Calcium signal modulators | EF hand family protein [Trichomonas vaginalis G3] (12) | 2 (2) | 70,33 | 2,55E+04 | 1,03E+05 | 4,06 | 4,41E-07 | gi|121885510 |
| Cellular metabolism | Calcium signal modulators | calmodulin, putative [Trichomonas vaginalis G3] (13) | 3 (3) | 202,21 | 1,01E+04 | 3,40E+04 | 3,36 | 5,73E-07 | gi|121905996 |
| Cellular metabolism | Calcium signal modulators | centrin, putative [Trichomonas vaginalis G3] (14) | 3 (3) | 83,18 | 1,15E+04 | 3,79E+04 | 3,29 | 1,69E-05 | gi|121913299 |
| Cellular metabolism | Membrane trafficking Signal transduction | C2 domain containing protein [Trichomonas vaginalis G3] (15) | 2 (2) | 36,07 | 7828,24 | 3,91E+04 | 4,99 | 5,52E-03 | gi|121897227 |
| Cellular metabolism | Membrane trafficking Rab GTPase binding Deaminase |
Vps52 / Sac2 family protein [Trichomonas vaginalis G3] (16) | 2 (2) | 29,81 | 4624,93 | 3,38E+04 | 7,31 | 1,77E-06 | gi|121902006 |
| Exocytosis | Vesicle mediated transport | SNARE protein, putative [Trichomonas vaginalis G3] (17) | 2 (2) | 94,78 | 1819,33 | 6479,78 | 3,56 | 2,51E-03 | gi|121914867 |
| Exocytosis | Vesicle-mediated transport Signal transduction |
CMGC family protein kinase [Trichomonas vaginalis G3] (18) | 2 (2) | 48,38 | 2,85E+04 | 1,00E+05 | 3,51 | 1,94E-03 | gi|121882186 |
| Endocytosis | Vesicle mediated transport | SacI homology domain containing protein [Trichomonas vaginalis G3] (19) | 2 (2) | 35,91 | 7081,01 | 8,59E+04 | 12,12 | 1,50E-05 | gi|121895312 |
| Cellular metabolism | Carbohydrate metabolic process | Malate dehydrogenase: SUBUNIT = A, putative [Trichomonas vaginalis G3] (20) | 4 (4) | 245,34 | 2,16E+05 | 1,41E+06 | 6,54 | 5,90E-06 | gi|121896029 |
| Cellular metabolism | Carbohydrate metabolic process | Malate dehydrogenase, putative [Trichomonas vaginalis G3] (21) | 3 (3) | 156,4 | 1,55E+05 | 5,36E+05 | 3,46 | 2,12E-05 | gi|121915536 |
| Cellular metabolism | Carbohydrate metabolic process Energetic metabolism | Starch binding domain containing protein [Trichomonas vaginalis G3] (22) | 3 (3) | 79,81 | 1,98E+04 | 1,33E+05 | 6,73 | 8,92E-04 | gi|121903627 |
| Cellular metabolism | Carbohydrate metabolic process Energetic metabolism | 4-alpha-glucanotransferase family protein [Trichomonas vaginalis G3] (23) | 10 (10) | 425,98 | 4,24E+04 | 1,30E+05 | 3,07 | 6,46E-07 | gi|121895827 |
| Cellular metabolism | Carbohydrate metabolic process Transferase-kinase |
pyruvate, phosphate dikinase family protein [Trichomonas vaginalis G3] (24) | 9 (9) | 562,62 | 1,32E+04 | 9,12E+04 | 6,9 | 1,36E-08 | gi|121904065 |
| Cellular metabolism | Carbohydrate metabolic process Glycolysis Transferase-kinase |
PEP-utilizing enzyme, TIM barrel domain containing protein [Trichomonas vaginalis G3] (25) | 2 (2) | 74,26 | 1978,28 | 2,01E+04 | 10,16 | 1,22E-03 | gi|121914977 |
| Cellular metabolism | Carbohydrate metabolic process Glycolysis |
enolase 4, putative [Trichomonas vaginalis G3] (26) | 3 (2) | 88,69 | 5589,54 | 2,28E+04 | 4,07 | 4,60E-06 | gi|121908406 |
| Cellular metabolism | Nucleobase-containing compound metabolic process Glycogen metabolic process Response to stress Transcription Metallophosphatase |
phosphatase, putative [Trichomonas vaginalis G3] (27) | 3 (3) | 110,32 | 6005,06 | 2,02E+04 | 3,37 | 2,59E-08 | gi|121888987 |
| Cellular metabolism | Energetic metabolism Nucleobase-containing compound metabolic process |
Phosphorylase family protein [Trichomonas vaginalis G3] (28) | 3 (3) | 94,45 | 9127,21 | 2,96E+04 | 3,24 | 1,04E-04 | gi|121891200 |
| Cellular metabolism | Peptidase | Clan SB, family S8, subtilisin-like serine peptidase [Trichomonas vaginalis G3] (29) | 2 (2) | 47,09 | 1,63E+04 | 9,69E+04 | 5,93 | 3,44E-04 | gi|121885232 |
| Cellular metabolism | Peptidase Cysteine protease Calmodulin |
Clan CA, family C2, calpain-like cysteine peptidase [Trichomonas vaginalis G3] (30) | 3 (3) | 44,02 | 3,07E+04 | 1,50E+05 | 4,88 | 3,37E-04 | gi|121907418 |
| Cellular metabolism | Oxidoreductase-peroxidase Response to stress |
hybrid-cluster protein, putative [Trichomonas vaginalis G3] (31) | 2 (2) | 32,17 | 1467,22 | 2,69E+04 | 18,33 | 8,26E-06 | gi|121908608 |
| Cellular metabolism | Protein metabolism Response to stress Chaperone |
DnaJ domain containing protein [Trichomonas vaginalis G3] (32) | 2 (2) | 61,26 | 2597,33 | 2,66E+04 | 10,22 | 6,84E-05 | gi|121911862 |
| Cellular metabolism | Protein synthesis Transcription factor |
NAC domain containing protein [Trichomonas vaginalis G3] (33) | 2 (2) | 125,14 | 2206,15 | 6,49E+04 | 29,42 | 2,64E-06 | gi|121897587 |
| Cellular metabolism | Protein synthesis Translation |
ribosomal protein L10a [Trichomonas vaginalis] (34) | 2 (2) | 136,77 | 4,43E+04 | 2,00E+05 | 4,52 | 2,10E-04 | gi|4193363 |
| Cellular metabolism | Protein synthesis | elongation factor 1 alpha, partial [Trichomonas vaginalis] (35) | 11 (11) | 526,1 | 4,75E+05 | 1,44E+06 | 3,02 | 9,55E-05 | gi|329750813 |
| Cellular metabolism | Protein-protein interaction Ribosyltransferase |
B-box zinc finger family protein [Trichomonas vaginalis G3] (36) | 2 (2) | 30,34 | 1,00E+04 | 5,62E+04 | 5,61 | 4,39E-09 | gi|121906335 |
| Cellular metabolism | Protein-protein interaction | erythrocyte binding protein, putative [Trichomonas vaginalis G3] (37) | 4 (4) | 181,54 | 2,41E+04 | 1,26E+05 | 5,22 | 1,63E-08 | gi|121898736 |
| DNA replication | DNA replication | XRN 5'-3' exonuclease N-terminus family protein [Trichomonas vaginalis G3] (38) | 2 (2) | 38,94 | 3998,07 | 2,45E+04 | 6,13 | 2,43E-04 | gi|121913977 |
| DNA replication | DNA replication DNA recombination and DNA repair Response to stress |
ATP-dependent DNA helicase, RecQ family protein [Trichomonas vaginalis G3] (39) | 2 (2) | 34,18 | 1,04E+04 | 3,74E+04 | 3,59 | 2,96E-04 | gi|121909374 |
| DNA replication | Nucleus regulation Chromatin replication |
SNF2 family N-terminal domain containing protein [Trichomonas vaginalis G3] (40) | 2 (2) | 28,25 | 1,23E+05 | 9,67E+05 | 7,86 | 1,25E-05 | gi|121903240 |
| Cellular component | Translation | SRP54-type protein, putative [Trichomonas vaginalis G3] (41) | 4 (4) | 103,41 | 3,00E+04 | 1,48E+05 | 4,91 | 3,56E-03 | gi|121902866 |
| Translation | Translation | eukaryotic release factor 3 GTPase subunit [Trichomonas vaginalis] (42) | 3 (3) | 116,13 | 3470,55 | 1,83E+04 | 5,27 | 7,97E-04 | gi|8307949 |
| Translation | Translation | translation initiation factor eIF-5A family protein [Trichomonas vaginalis G3] (43) | 2 (2) | 108,14 | 6,24E+04 | 2,49E+05 | 3,99 | 2,43E-05 | gi|121876621 |
Identified and relatively quantified proteins from membrane proteins enriched fraction for T. gallinae clonal cultures more abundant in clone R17-12 C1. Proteins were grouped by category, protein function and fold change. Only proteins with 2 or more identified peptides, ≥ 3 fold change and statistical significance (ANOVA, p-value < 0.01) are listed.
1 cn: cytoscape reference number in Fig 4.
2 P: number of identified peptides.
3 (n): number of unique, non-conflicting peptides.
4 total protein score (sum of individual peptides scores).