Table 4. Proteins more abundant in P178.
| Category | Predicted Functions | Description (cn)1 | P2 (n)3 | Score4 | Clone P178-13 C7 | Clone R17-12 C1 | Fold Change | ANOVA p Value | Accession Number |
|---|---|---|---|---|---|---|---|---|---|
| Cellular component | Cytoskeleton | profilin, putative [Trichomonas vaginalis G3] (1) | 2 (2) | 64,27 | 6,67E+04 | 1,40E+04 | 4,75 | 2,17E-05 | gi|121906940 |
| Cellular component | Cytoskeleton Metal ion binding |
putative alpha-actinin [Histomonas meleagridis] (2) | 3 (3) | 48,12 | 7720,57 | 1239 | 6,23 | 2,59E-03 | gi|262358541 |
| Signal transduction | Signal transduction | small GTP-binding protein, putative [Trichomonas vaginalis G3] (3) | 2 (2) | 237,3 | 3,06E+05 | 7,28E+04 | 4,21 | 1,97E-03 | gi|121880468 |
| Signal transduction | Signal transduction | GTPase-activator protein, putative [Trichomonas vaginalis G3] (4) | 2 (2) | 29,36 | 2,40E+05 | 6,07E+04 | 3,95 | 3,09E-04 | gi|121892626 |
| Cellular metabolism | Response to stress | Heat shock 70 kDa protein, mitochondrial precursor, putative [Trichomonas vaginalis G3] (5) | 2 (2) | 74,39 | 2,02E+05 | 2,48E+04 | 8,13 | 1,66E-03 | gi|121914923 |
| Cellular metabolism | Response to stress | Hsp90 protein [Trichomonas vaginalis G3] (6) | 6 (6) | 326,25 | 3,83E+04 | 1,19E+04 | 3,22 | 1,97E-05 | gi|121917639 |
| Cellular metabolism | Oxidation-reduction homeostasis Vesicle-mediated transport Small GTPase regulator (-) Signal transduction |
GDP dissociation inhibitor family protein [Trichomonas vaginalis G3] (7) | 6 (6) | 355,73 | 1,37E+05 | 3,96E+04 | 3,46 | 4,89E-05 | gi|121879717 |
| Endocytosis | Vesicle-mediated transport Cytoskeleton Small GTPase |
Dynamin central region family protein [Trichomonas vaginalis G3] (8) | 3 (3) | 64,69 | 5,79E+04 | 7821,44 | 7,4 | 5,98E-05 | gi|121887114 |
| Exocytosis | Vesicle-mediated transport | Clathrin and VPS domain-containing protein, partial [Trichomonas vaginalis G3] (9) | 8 (8) | 349,57 | 1,08E+05 | 1,51E+04 | 7,17 | 1,06E-04 | gi|121873597 |
| Exocytosis | Vesicle-mediated transport | Clathrin and VPS domain-containing protein [Trichomonas vaginalis G3] (10) | 6 (6) | 208,37 | 1,46E+05 | 2,06E+04 | 7,11 | 2,48E-05 | gi|121898439 |
| Cellular metabolism | Transmembrane transport | ABC transporter family protein [Trichomonas vaginalis G3] (11) | 4 (4) | 149,49 | 2,76E+04 | 5066,61 | 5,45 | 3,12E-07 | gi|121883001 |
| Cellular metabolism | Transmembrane transport | ABC transporter family protein [Trichomonas vaginalis G3] (12) | 3 (3) | 99,04 | 3,27E+04 | 7707,22 | 4,24 | 7,94E-05 | gi|121889755 |
| Cellular metabolism | Transmembrane transport | hydrogenosomal carrier protein, partial [Trichomonas gallinae] (13) | 14 (14) | 744,55 | 2,07E+06 | 5,02E+05 | 4,12 | 5,36E-04 | gi|30315255 |
| Cellular metabolism | Transmembrane transport | phospholipid-translocating P-type ATPase, flippase family protein [Trichomonas vaginalis G3] (14) | 2 (2) | 49,93 | 1,47E+05 | 3,84E+04 | 3,81 | 1,56E-05 | gi|121897029 |
| Cellular metabolism | Carbohydrate metabolic process Energetic metabolism |
lactate dehydrogenase family protein [Trichomonas vaginalis G3] (15) | 2 (2) | 43,1 | 1,21E+05 | 5473,69 | 22,13 | 3,85E-07 | gi|121889193 |
| Cellular metabolism | Carbohydrate metabolic process Energetic metabolism |
glucose-6-phosphate 1-dehydrogenase family protein [Trichomonas vaginalis G3] (16) | 7 (7) | 285,86 | 8,47E+04 | 1,21E+04 | 7 | 2,95E-07 | gi|121883413 |
| Cellular metabolism | Carbohydrate metabolic process Gluconeogenesis Glycolysis |
glucose-6-phosphate isomerase family protein [Trichomonas vaginalis G3] (17) | 17 (17) | 917,8 | 2,76E+05 | 5,44E+04 | 5,08 | 1,37E-04 | gi|121906378 |
| Cellular metabolism | Carbohydrate metabolic process Energetic metabolism |
glucose-6-phosphate isomerase family protein [Trichomonas vaginalis G3] (18) | 3 (3) | 89,42 | 7,67E+04 | 1,71E+04 | 4,49 | 1,22E-06 | gi|121908707 |
| Cellular metabolism | Carbohydrate metabolic process Energetic metabolism |
galactokinase family protein [Trichomonas vaginalis G3] (19) | 3 (3) | 134,69 | 2,73E+04 | 6100,32 | 4,47 | 3,28E-07 | gi|121903545 |
| Cellular metabolism | Carbohydrate metabolic process Energetic metabolism |
Alpha amylase, catalytic domain containing protein [Trichomonas vaginalis G3] (20) | 2 (2) | 94,07 | 1,35E+04 | 3431,11 | 3,94 | 9,70E-04 | gi|121915021 |
| Cellular metabolism | Carbohydrate metabolic process Energetic metabolism |
glycosyl transferase, group 1 family protein [Trichomonas vaginalis G3] (21) | 2 (2) | 43,52 | 2,36E+04 | 6251,76 | 3,78 | 5,41E-05 | gi|121889369 |
| Cellular metabolism | Carbohydrate metabolic process Energetic metabolism |
6-phosphogluconate dehydrogenase, decarboxylating family protein [Trichomonas vaginalis G3] (22) | 2 (2) | 99,56 | 1,10E+05 | 3,03E+04 | 3,64 | 6,83E-06 | gi|121896682 |
| Cellular metabolism | Carbohydrate metabolic process Metal ion binding Lyase |
fructose-1,6-bisphosphate aldolase, putative [Trichomonas vaginalis G3] (23) | 12 (12) | 714,5 | 1,64E+06 | 5,03E+05 | 3,26 | 2,12E-03 | gi|121909882 |
| Cellular metabolism | Protein metabolism cysteine peptidase |
Clan CA, family C1, cathepsin L-like cysteine peptidase [Trichomonas vaginalis G3] (24) | 2 (2) | 43,69 | 2,27E+04 | 4950,94 | 4,59 | 3,18E-03 | gi|121908100 |
| Cellular metabolism | Protein metabolism Peptidase |
Clan CA, family C1, cathepsin L-like cysteine peptidase, partial [Trichomonas gallinae] (25) | 6 (6) | 286,99 | 1,28E+06 | 3,49E+05 | 3,67 | 1,69E-03 | gi|386364444 |
| Cellular metabolism | Protein metabolism metalloprotease |
Clan MH, family M20, peptidase T-like metallopeptidase [Trichomonas vaginalis G3] (26) | 3 (3) | 123,12 | 1,34E+05 | 2,97E+04 | 4,49 | 1,24E-04 | gi|121901617 |
| Cellular metabolism | Protein metabolism threonine peptidase |
Family T1, proteasome beta subunit, threonine peptidase [Trichomonas vaginalis G3] (27) | 3 (3) | 94,65 | 3,75E+04 | 1,24E+04 | 3,03 | 1,47E-04 | gi|121916758 |
| Cellular metabolism | Protein metabolism protein kinases |
CAMK family protein kinase [Trichomonas vaginalis G3] (28) | 2 (2) | 31,32 | 8977,61 | 715,69 | 12,54 | 5,04E-03 | gi|121906466 |
| Cellular metabolism | Protein metabolism protein kinases |
CAMK family protein kinase [Trichomonas vaginalis G3] (29) | 2 (2) | 32,25 | 1,62E+04 | 1619,25 | 9,98 | 1,01E-03 | gi|121912207 |
| Cellular metabolism | Protein metabolism Protein kinase Signal transduction |
AGC family protein kinase [Trichomonas vaginalis G3] (30) | 2 (2) | 54,75 | 1,69E+05 | 3,65E+04 | 4,63 | 9,97E-03 | gi|121890243 |
| Cellular metabolism | Metal ion binding Oxidation-reduction homeostasis |
putative NADPH-dependent butanol dehydrogenase [Trichomonas vaginalis] (31) | 2 (2) | 206,35 | 4,42E+04 | 2102,87 | 21,01 | 2,18E-05 | gi|4426910 |
| Cellular metabolism | Metal ion binding Oxidation-reduction homeostasis |
Pyridine nucleotide-disulphide oxidoreductase family protein [Trichomonas vaginalis G3] (32) | 3 (3) | 178,07 | 2,07E+05 | 3,00E+04 | 6,9 | 9,64E-08 | gi|121898098 |
| Cellular metabolism | Oxidation-reduction homeostasis | thioredoxin-disulfide reductase family protein [Trichomonas vaginalis G3] (33) | 2 (2) | 113,87 | 1,21E+05 | 2,93E+04 | 4,14 | 1,62E-03 | gi|121893127 |
| Cellular metabolism | Protein metabolism | Glutamine synthetase, catalytic domain containing protein [Trichomonas vaginalis G3] (34) | 3 (3) | 107,13 | 7,43E+04 | 9390,65 | 7,91 | 1,67E-08 | gi|121892813 |
| Cellular metabolism | Protein metabolism | aminotransferase, classes I and II family protein [Trichomonas vaginalis G3] (35) | 2 (2) | 164,19 | 1,55E+05 | 4,54E+04 | 3,41 | 3,92E-05 | gi|121909674 |
| Cellular metabolism | Protein metabolism | Copine family protein [Trichomonas vaginalis G3] (36) | 3 (3) | 53,44 | 6,76E+04 | 2,14E+04 | 3,16 | 2,31E-03 | gi|121901567 |
| Cellular metabolism | Protein metabolism | co-chaperone Hsc20 family protein [Trichomonas vaginalis G3] (37) | 2 (2) | 70,46 | 3,19E+04 | 1,03E+04 | 3,11 | 0,01 | gi|121889907 |
| Cellular metabolism | Protein metabolism Phospholipid metabolic process |
glycosyl transferase, group 2 family protein [Trichomonas vaginalis G3] (38) | 4 (4) | 195,85 | 2,82E+04 | 4751,28 | 5,94 | 1,67E-05 | gi|121911637 |
| Cellular metabolism | Energetic metabolism Protein glycosylation |
Oligosaccharyl transferase STT3 subunit family protein [Trichomonas vaginalis G3] (39) | 2 (2) | 63,86 | 1,33E+04 | 323,32 | 41,08 | 7,69E-08 | gi|121902670 |
| Cellular metabolism | Glycogen metabolic process Transferase |
UTP-glucose-1-phosphate uridylyltransferase family protein [Trichomonas vaginalis G3] (40) | 5 (5) | 168,95 | 2,17E+04 | 6092,98 | 3,56 | 2,44E-06 | gi|121904554 |
| Cellular metabolism | Translation Transmembrane transport Calmodulin Ribosomal protein Mitocondrial carrier protein |
Mitochondrial carrier protein [Trichomonas vaginalis G3] (41) | 9 (9) | 479,27 | 6,94E+05 | 1,77E+05 | 3,92 | 4,36E-04 | gi|121889868 |
| Cellular component | Ribosomal protein RNA metabolic process Protein metabolic processes |
40S ribosomal protein S16, putative [Trichomonas vaginalis G3] (42) | 2 (2) | 143,1 | 5,03E+04 | 1,45E+04 | 3,47 | 7,03E-04 | gi|121885138 |
| Cellular metabolism | Energetic metabolism Protein complex assembly HSP70/actin superfamily |
dnaK protein [Trichomonas vaginalis G3] (43) | 4 (4) | 101,55 | 1,64E+05 | 1,84E+04 | 8,93 | 5,84E-08 | gi|121907663 |
| Cellular metabolism | Transaldolase | Transaldolase A, putative [Trichomonas vaginalis G3] (44) | 2 (2) | 62,98 | 2,40E+04 | 4347,89 | 5,52 | 8,56E-05 | gi|121896456 |
| Cellular metabolism | Transaminase Cellular aminoacid metabolic process |
aminotransferase, classes I and II family protein [Trichomonas vaginalis G3] (45) | 3 (3) | 158,74 | 1,17E+05 | 2,72E+04 | 4,3 | 9,78E-03 | gi|121901152 |
| Cellular metabolism | Transcription | RNA polymerase II largest subunit, partial [Trichomonas gallinae] (46) | 2 (2) | 87,66 | 8257,66 | 1452,08 | 5,69 | 2,60E-04 | gi|327242119 |
| Cellular metabolism | Transcription | histone H4-3 [Trichomonas vaginalis G3] (47) | 2 (2) | 184,78 | 2,99E+05 | 9,01E+04 | 3,32 | 3,19E-03 | gi|121889898 |
| Cellular component | Hydrogenase, metal ion binding | hydrogenosomal Fe-hydrogenase, partial [Trichomonas gallinae] (48) | 10 (10) | 704,23 | 2,57E+05 | 7,19E+04 | 3,58 | 8,81E-03 | gi|507310429 |
| Cellular metabolism | Energetic metabolism Nucleobase containing compound metabolic process Signal transduction |
3'5'-cyclic nucleotide phosphodiesterase family protein [Trichomonas vaginalis G3] (49) | 3 (3) | 60,29 | 4,57E+05 | 1,29E+05 | 3,54 | 7,28E-04 | gi|121889147 |
| Cellular metabolism | ATPase Calcium signal modulators Nucleobase-containing compound metabolic process Transmembrane calcium ion transport |
calcium-translocating P-type ATPase, PMCA-type family protein [Trichomonas vaginalis G3] (50) | 2 (2) | 71,75 | 5,25E+04 | 8685,17 | 6,04 | 2,13E-05 | gi|121889617 |
| Cellular metabolism | ATPase | ATPase, AAA family protein [Trichomonas vaginalis G3] (51) | 2 (2) | 30,29 | 5,54E+05 | 3,85E+04 | 14,38 | 1,45E-06 | gi|121886218 |
| Cellular metabolism | Metal ion binding Transferase | Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I family protein [Trichomonas vaginalis G3] (52) | 8 (8) | 414,71 | 2,67E+05 | 7,70E+04 | 3,47 | 3,13E-05 | gi|121885206 |
| Cellular metabolism | DNA replication Energetic metabolism |
ATP-dependent DNA helicase, RecQ family protein [Trichomonas vaginalis G3] (53) | 3 (2) | 54,81 | 2,05E+05 | 6,37E+04 | 3,22 | 2,82E-03 | gi|121888663 |
| DNA replication | DNA replication | polymerase zeta subunit, putative [Trichomonas vaginalis G3] (54) | 2 (2) | 36,13 | 2,96E+04 | 6239,31 | 4,74 | 1,58E-06 | gi|121909895 |
| DNA replication | DNA replication | helicase, putative [Trichomonas vaginalis G3] (55) | 2 (2) | 36,9 | 4,43E+04 | 9427,08 | 4,7 | 2,77E-07 | gi|121911876 |
| DNA replication | DNA replication Transcription |
possible regulator of nonsense transcripts, putative [Trichomonas vaginalis G3] (56) | 3 (3) | 81,75 | 4,87E+05 | 3,46E+04 | 14,09 | 7,25E-07 | gi|121916893 |
| DNA replication | DNA replication Transcription |
Ribonucleotide reductase, all-alpha domain containing protein [Trichomonas vaginalis G3] (57) | 5 (5) | 255,06 | 1,61E+05 | 3,94E+04 | 4,09 | 2,53E-04 | gi|121915211 |
| DNA replication | Nucleotide binding | heavy neurofilament protein, putative [Trichomonas vaginalis G3] (58) | 3 (3) | 46,16 | 1,54E+04 | 4756,37 | 3,23 | 5,43E-05 | gi|121898592 |
| Translation | RNA metabolic process | Type III restriction enzyme, res subunit family protein [Trichomonas vaginalis G3] (59) | 2 (2) | 46,03 | 7,36E+05 | 2,01E+05 | 3,66 | 8,63E-03 | gi|121898850 |
| Translation | Metal ion binding | alanyl-tRNA synthetase, partial [Trichomonas vaginalis] (60) | 5 (5) | 187,01 | 1,31E+05 | 1,71E+04 | 7,63 | 8,44E-06 | gi|51102334 |
Identified and relatively quantified proteins from membrane proteins enriched fraction for T. gallinae clonal cultures more abundant in clone P178-13 C7. Proteins were grouped by category, protein function and fold change. Only proteins with 2 or more identified peptides, ≥ 3 fold change and statistical significance (ANOVA, p-value < 0.01) are listed.
1 cn: cytoscape reference number in Fig 5
2 P: number of identified peptides.
3 (n): number of unique, non-conflicting peptides.
4 total protein score (sum of individual peptides scores).