Fig 5. Centromeric SMC1 is significantly reduced in Mcm5^A7 mutants.

a. Representative images of chromosome spreads in WT and Mcm5^A7 meiotic nuclei examining localization of SMC1 (magenta) and CID (green). Green arrow: SMC1 enrichment at the centromere, yellow arrow and tract: SMC1 along the chromosome arm. Scale bar = 2 μm. Contrast and brightness of images were adjusted for clarity. b. Integrated SMC1 fluorescence intensity at CID foci in meiotic nuclei at meiotic onset (zygotene + early pachytene, Region 2A) at WT (n = 225) and Mcm5^A7 (n = 398) meiotic centromeres. ***p < 0.0001, unpaired T-test. Data are represented as mean ± SD. c. CID-localized SMC1 fluorescence intensity subtracted from total nuclear SMC1 fluorescence in meiotic nuclei at meiotic onset (zygotene + early pachytene, Region 2A) in WT (n = 81) and Mcm5^A7 (n = 93) meiotic nuclei. n.s. = 0.0548, unpaired T-test. Data are represented as mean ± SD. S3A Fig for representative images. d. Mcm5^A7 (n = 1194) and ord¹⁰ (n = 250) mutants examined for inter-sister recombination through the ratio of Ring chromosome to Rod chromosome transmission. WT (n = 2574 for Mcm5^A7 experiment, n = 1204 for Ord experiment) was normalized to 1. Ratios above 1 suggest less inter-sister recombination; ratios below 1 suggest more inter-sister recombination. Refer to S1 Table for complete dataset. For further details about the assay, see S3B–S3D Fig.