Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-TPH2 (rabbit polyclonal) | Novus | Cat# NB100-74555 |
IF (1:1000) |
| Antibody | anti-GFP (chicken polyclonal) | Aves Labs Inc | Cat# GFP-1020 | IF (1:2000); iDISCO (1:1000) |
| Antibody | anti-rabbit donkey antibody conjugated with Cy3 | Jackson ImmunoResearch | Cat# 711-165-152 |
1:500 |
| Antibody | anti-rabbit donkey antibody conjugated with Cy5 | Jackson ImmunoResearch | Cat# 711-496-152 |
1:500 |
| Antibody | anti-chicken donkey antibody conjugated with Cy2 | Jackson ImmunoResearch | Cat# 703-605-155 |
1:500 |
| Recombinant DNA reagent |
pAAV-Ef1a-fDIO-EYFP
(plasmid) |
Addgene | Cat# 27437 | |
| Recombinant DNA reagent | STOPx3 (plasmid) | Addgene | Cat# 22799 | |
| Recombinant DNA reagent |
Membrane tag
(plasmid) |
Addgene | Cat # 71760 | |
| Commercial assay or kit | Gibson Assembly Master Mix |
New England Biolabs | Cat# E2611S | |
| Commercial assay, kit | Papain Dissociation System | Worthington | Cat# LK003150 | |
| Commercial assay, kit | C1ql2 | Molecular Instruments | NM_207233.1 | B1 amplifierv3.0 probe |
| Commercial assay, kit | Crhr2 | Molecular Instruments | NM_001288620.1 | B1 amplifierv3.0 probe |
| Commercial assay, kit | Dlk1 | Molecular Instruments | NM_001190703.1 | B2 amplifierv3.0 probe |
| Commercial assay, kit | Ret | Molecular Instruments | NM_009050.2 | B4 amplifierv3.0 probe |
| Commercial assay, kit | Gad1 | Molecular Instruments | NM_008077.5 | B1 amplifierv3.0 probe |
| Commercial assay, kit | Gad2 | Molecular Instruments | NM_008078.2 | B1 amplifierv3.0 probe |
| Commercial assay, kit | Spp1 | Molecular Instruments | NM_001204202.1 | B4 amplifierv3.0 probe |
| Commercial assay, kit | Syt2 | Molecular Instruments | NM_001355726.1 | B1 amplifierv3.0 probe |
| Commercial assay, kit | Tacr3 | Molecular Instruments | NM_021382.6 | B1 amplifierv3.0 probe |
| Commercial assay, kit | Irx2 | Molecular Instruments | NM_010574.4 | B1 amplifierv3.0 probe |
| Commercial assay, kit | Npas1 | Molecular Instruments | NM_008718.2 | B4 amplifierv3.0 probe |
| Commercial assay, kit | Piezo2 | Molecular Instruments | NM_001039485.4 | B1 amplifierv3.0 probe |
| Commercial assay, kit | Tpbg | Molecular Instruments | NM_001164792.1 | B4 amplifierv3.0 probe |
| Commercial assay, kit | Met | Molecular Instruments | NM_008591.2 | B4 amplifierv3.0 probe |
| Commercial assay, kit | Trh | Molecular Instruments | NM_009426.3 | B2 amplifierv3.0 probe |
| Commercial assay, kit | Vglut3 | Molecular Instruments | NM_001310710.1 | B4 amplifierv3.0 probe |
| Commercial assay, kit |
TPH2 | Molecular Instruments | NM_173391 | B3 amplifierv3.0 probe |
| Chemical compound, drug | Tetrodotoxin | Tocris Bioscience | Cat# 1069 | 1 μM |
| Chemical compound, drug | Kynurenic acid | Millipore Sigma | Cat# K3375 | 500 μM |
| Chemical compound, drug |
D-AP5 | Tocris Bioscience | Cat# 0106 | 50 μM |
| Chemical compound, drug | Actinomycin D | Sigma | Cat# A1410 | 50 μM |
| Software, algorithm | IMARIS | Bitplane | RRID:SCR_007370 | Bitplane.com |
| Software, algorithm | Ilastik | GNU General Public License | RRID:SCR_015246 | https://ilastik.org/ |
| Software, algorithm | Elastix | Image Sciences Institute | RRID:SCR_009619 | https://elastix.isi.uu.nl/ |
| Software, algorithm | MATLAB | Mathworks | RRID:SCR_001622 | Mathworks.com |
| Software, algorithm | Fiji | PMID: 22743772 | RRID:SCR_002285 | https://imagej.net/Fiji |
| Software, algorithm | Allen Institute’sCommon Coordinate Framework (CCF) | Allen Institute for Brain Science (https://www.alleninstitute.org/) | https://download.alleninstitute.org/informatics-archive/current-release/mouse_ccf/ | |
| Software | R v3.5.3 | R-project | RRID:SCR_001905 | |
| Software | Seurat v3.0 | https://github.com/satijalab/seurat | RRID: SCR_016341 | Butler et al., 2018; Stuart et al., 2019 |
| Software | STAR 2.6.1a | https://github.com/alexdobin/STAR | RRID:SCR_015899 | Dobin et al., 2013 |
| Software | HTseq 0.11.2 | European Molecular Biology Laboratory | RRID:SCR_005514 | Anders et al., 2015 |
| Other | Normal donkey serum | Jackson ImmunoResearch | Cat# 017-000-121 | |
| Other | DAPI stain | Invitrogen | Cat# D1306 | (1 µg/mL) |
| Other | SH800S FACS sorter | SONY | ||
| Other | NextSeq500 | Illumina | https://www.illumina.com |