TABLE 2.
Limits of detection for rifampin heteroresistance among triplicatesa
| Strain ID | Rifampin resistance-conferring mutations | Values by RDT quantified by Deeplex |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| LPA-Hain (%LOD)1 | Deeplex (%QP)1 | LPA-Nipro (%LOD)1 | Deeplex (%QP)1 | Xpert (%LOD)1 | Deeplex (%QP)1 | Ultra (%LOD)2 | Deeplex (%QP)2 | |||
| TB-TDR-0036 | H445Db | H526Dc | 5–10 | 7.5 | 5 | 7.5 | 40–60 | 55.9 | 40–60 | |
| TB-TDR-0090 | S450Lb | S531Lc | 5 | 5 | 1–5 | 5 | 20–40 | 21.7 | 20–30 | 21.7 |
| TB-TDR-0100 | D435Vb | D516Vc | 5–10 | 6 | 5–10 | 6 | 70–80 | 69.6 | 40–50 | |
| TB-TDR-0131 | H445Yb | H526Yc | 5–10 | 4.3 | 10 | 8.7 | 70–80 | 66.7 | 60–70 | |
a
With quantification of the respective lowest proportion by targeted deep sequencing (Deeplex-MycTB). ID, identifier; Deeplex-MycTB, targeted deep sequencing; RDT, rapid diagnostic test; LPA-Hain, GenoTypeMTDBRplusv2.0; LPA-Nipro, GenoscholarNTM+MTBII; Xpert, classic XpertMTB/RIF; Ultra, Xpert MTB/RIF Ultra; 1, batch 1; 2, batch 2; %LOD, limit of detection (range) among triplicates for the RDTs; %QP, quantified proportion of minority resistant variants determined by Deeplex on replicate 3.
b
In M. tuberculosis numbering.
c
In E. coli numbering.