TABLE 4.
Performance of MALDI-TOF MS for identification of S. delphini
| Isolate identifier | Group | MALDI Biotyper full extraction preparative method |
Vitek MS direct application preparative method |
|||
|---|---|---|---|---|---|---|
| IVD database |
RUO database identification (log [score] value)a | IVD database non-clinically validated identificationb (confidence value [%])c | RUO database identification (confidence value [%])c | |||
| Clinically validated identification (log [score] value)a | Non-clinically validated identification (log [score] value)a | |||||
| DSM 20771T | A | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| MI 09-2894 | A | No identification (<1.70) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| MI 09-8445 | A | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| MI 16-1129 | A | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| VPP 12 D | A | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| 19-039 | A | No identification (<1.70) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (48.2%), S. intermedius (51.7%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| 52-006 | A | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| 56-021 | A | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| 62-031 | A | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| 8086 | B | S. pseudintermedius (≥2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| AV8047 | B | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| H4A | B | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (50%), S. intermedius (49.9%) | S. delphini/S. intermedius/S. pseudintermedius (92.1%) |
| HT2003-0674 | B | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| P27B | B | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (50%), S. intermedius (50%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| P50 | B | S. pseudintermedius (<2.00) | S. delphini (<2.00) | S. delphini (≥2.00) | S. pseudintermedius (45%), S. intermedius (54.9%) | S. delphini/S. intermedius/S. pseudintermedius (76.1%) |
| 26-037 | B | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| 33-090 | B | No identification (<1.70) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| 35-016 | B | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (50%), S. intermedius (50%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| 49-046 | B | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (88.1%) |
| 53-033 | B | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (99.9%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
| 56-039 | B | S. pseudintermedius (<2.00) | S. delphini (≥2.00) | S. delphini (≥2.00) | S. pseudintermedius (49.2%), S. intermedius (50.8%) | S. delphini/S. intermedius/S. pseudintermedius (99.9%) |
a
Log (score) value of 2.00 to 3.00, high-confidence identification; log (score) value of 1.70 to1.99, low-confidence identification; log (score) value of <1.70, no organism identification possible.
b
No member of the SIG was included in the clinically validated Vitek MS IVD Knowledge Base v2.0 database; however, S. intermedius and S. pseudintermedius were included as non-clinically validated species in the IVD Knowledge Base v2.0 database.
c
Confidence value of 60.0 to 99.9%, high-confidence identification (one significant organism or organism group identification returned); confidence value of <60%, low-discrimination identification. Low discrimination identifications are displayed when more than one, but not more than four, organism or organism group identifications are returned.