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. 2019 Oct 23;19:994. doi: 10.1186/s12885-019-6179-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Breast cancer tissue quality evaluation and lncEPCAM/BC200 expression in breast cancer tissues. a H&E staining of breast cancer tissues. Expected tissue structures and morphology for normal tissue (left panel - ducts and ductules) and tumor tissue (right panel) (100x magnification). b Expression of BC200 in cancer tissue. BC200 is upregulated in 5 out of 10 patients’ breast tumor compared to normal adjacent tissue (BC200 is not expressed in the other 5 tumor tissues). Fold change was determined by the following equations: ΔCt = Ct_gene – Ct18S; ΔΔCt = ΔCt_gene – ΔCt_GFP; Fold change = 2^(−ΔΔCt) where 18S was used as housekeeping gene. Error bars indicate standard deviation between three technical replicates