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. 2019 Oct 23;19:994. doi: 10.1186/s12885-019-6179-y

Fig. 7.

Fig. 7

LncEPCAM locus. a Genomic region around lncEPCAM. NCBI representation of lncEPCAM/BC200/BCYRN1 genomic neighborhood. CALM2, EPCAM and MSH2 were selected to be further evaluated. b Evaluating Cis regulation. Effect of lncEPCAM/BC200 overexpression on nearby genes in MDA-MB-231 (MDA) and T47D cell lines. Fold change was determined by the following equations: ΔCt = Ct_gene – Ct18S; ΔΔCt = ΔCt_gene – ΔCt_GFP; Fold change = 2(−ΔΔCt) where 18S was used as housekeeping gene and Ct_GFP corresponds to threshold of the gene in cells that express GFP. Error bars indicate standard deviation between two independent experiments. MDA: MDA-MB-231; T-47D: T-47D