Fig. 7.

Comparison of the scalability of the upstream process from a 500-mL ventilated spinner flask to the 5-L bioreactor system. High Five™ cells were cultured in a 500-mL ventilated spinner flask and a 5-L bioreactor system, and the DO concentration was maintained at 80% throughout the production period. Once the Sf-21 cell density reached 2 × 10⁶ cells/mL, the cells were infected with the baculovirus. The cell growth curve (a), the H7N9-TW VLP production profile (b), and the glucose utilization profile (c) were monitored during the upstream process