Fig. 2. MALAT1 attenuates miR-145 expression by target binding. (A) According to starBase, there was a conserved complementary binding site of miR-145 in MALAT1. The mutant of MALAT1 wild-type (MALAT1-WT) is presented and named as MALAT1-MUT. (B and C) Transfection of miR-145 mimics/inhibitors distinctly decreased/facilitated the luciferase activity of MALAT1-WT chondrocytes. Expression level of MALAT1 and miR-145 in chondrocytes transfected with (D and E) siRNAs against MALAT1 (si-MALAT1 #1 and 2) or (F and G) pcDNA3.1-MALAT1 (MALAT1) vector. Data represent means±SD. *p<0.05.
