Fig. 3. MALAT1 modulates IL-1β-induced chondrocytes cell viability and cartilage ECM degradation via miR-145 in IL-1β-induced chondrocytes. (A and B) Chondrocytes were treated with different concentrations of IL-1β for 24 h, and expression of MALAT1 and miR-145 was detected using qRT-PCR. (C and D) Cell viability was measured by MTT assay in IL-1β-induced chondrocytes when transfected with si-MALAT1 #1 and MALAT1 vector only or combined with miR-145 mimics. (E) Expression of ECM-related proteins was examined by Western blotting in chondrocytes transfected with si-MALAT1 #1 and MALAT1 vector. (F and G) Quantitative analysis of ADAMTS5, COL2A1, ACAN, and COMP expressions using Image J. (H and I) Expression of ADAMTS5, COL2A1, ACAN, and COMP in IL-1β-induced chondrocytes transfected with MALAT1 vector only or combined with miR-145 mimics. All data are presented as means±SD. *p<0.05. ECM, extracellular matrix.
