Fig. 6. ADAMTS5 modulates IL-1β-induced chondrocyte viability and cartilage degradation mediated by MALAT1/miR-145. Chondrocytes were transfected with MALAT1 only or together with miR-145 mimics and miR-145 mimics only or combined with ADAMTS5 vector. After 10 ng/mL of IL-1β treatment, (A) cell viability was detected by MTT assay at 12 h, 24 h, 48 h, and 72 h, and (B) ECM-related protein expression was measured by Western blotting at 24 h. (C and D) Quantitative analysis of ADAMTS5, COL2A1, ACAN, and COMP expression was conducted by Image J. All data are presented as means±SD. ^*p<0.05.